Overexpression of Delta 12-Fatty Acid Desaturase in the Oleaginous Yeast Rhodosporidium toruloides for Production of Linoleic Acid-Rich Lipids

	Overexpression of Delta 12-Fatty Acid Desaturase in the Oleaginous Yeast Rhodosporidium toruloides for Production of Linoleic Acid-Rich Lipids
	Wang, Yanan 1,3; Zhao, Zongbao K.1; Jiao, Xiang 1; Yang, Xiaobing 1; Li, Li 2; Sun, Wenyi 1; Potter, Markus 2; Zhang, Sufang 1
刊名	APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
	2016-12-01
卷号	180 期号:8 页码:1497-1507
关键词	Rhodosporidium toruloides Delta 12-fatty acid desaturase Linoleic acid Microbial lipids Genetic engineering
英文摘要	Under nutrient-limited conditions, the red yeast Rhodosporidium toruloides can accumulate neutral lipids, of which the compositional fatty acids are mainly saturated and mono-unsaturated ones with 16 or 18 carbon atoms. To improve the linoleic acid content in the lipids, we enabled galactose-inducible expression of the gene encoding Delta 12-fatty acid desaturase (FADS) from Mortierella alpina or Fusarium verticillioides by integration of the corresponding expression cassettes into the genome of R. toruloides haploid and diploid strains. The relative linoleic acid content increased up to fivefold and the final linoleic acid titer reached 1.3 g/L under flask culture conditions. Our results suggested that R. toruloides may be further explored as cell factory for production of high-valued lipids and other fatty acid derivatives as bio-based chemicals and fuels.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]	SACCHAROMYCES-CEREVISIAE ; YARROWIA-LIPOLYTICA ; HETEROLOGOUS EXPRESSION ; TRANSFORMATION ; GENES ; IDENTIFICATION ; AGROBACTERIUM ; ACCUMULATION ; TOLERANCE ; CLONING
收录类别	SCI
语种	英语
WOS记录号	WOS:000389892200003
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/151823]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, 457 Zhongshan Rd, Dalian 116023, Peoples R China 2.Evon Degussa China Co Ltd, Shanghai Branch, Shanghai 201108, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yanan,Zhao, Zongbao K.,Jiao, Xiang,et al. Overexpression of Delta 12-Fatty Acid Desaturase in the Oleaginous Yeast Rhodosporidium toruloides for Production of Linoleic Acid-Rich Lipids[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2016,180(8):1497-1507.
APA	Wang, Yanan.,Zhao, Zongbao K..,Jiao, Xiang.,Yang, Xiaobing.,Li, Li.,...&Zhang, Sufang.(2016).Overexpression of Delta 12-Fatty Acid Desaturase in the Oleaginous Yeast Rhodosporidium toruloides for Production of Linoleic Acid-Rich Lipids.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,180(8),1497-1507.
MLA	Wang, Yanan,et al."Overexpression of Delta 12-Fatty Acid Desaturase in the Oleaginous Yeast Rhodosporidium toruloides for Production of Linoleic Acid-Rich Lipids".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 180.8(2016):1497-1507.