A novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells

	A novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells
	Liu, Tingjiao 1; Kong, Jing 1; Luo, Yong 2; Jin, Dong 1; An, Fan 2; Zhang, Wenyuan 1; Liu, Lilu 1; Li, Jiao 1; Fang, Shimeng 1; Li, Xiaojie 1
刊名	ONCOTARGET
	2016-11-29
卷号	7 期号:48 页码:78421-78432
关键词	microfluidic metastasis circulating tumor cells multi-organ bionic model
英文摘要	A biomimetic microsystem might compensate costly and time-consuming animal metastatic models. Herein we developed a biomimetic microfluidic model to study cancer metastasis. Primary cells isolated from different organs were cultured on the microlfuidic model to represent individual organs. Breast and salivary gland cancer cells were driven to flow over primary cell culture chambers, mimicking dynamic adhesion of circulating tumor cells (CTCs) to endothelium in vivo. These flowing artificial CTCs showed different metastatic potentials to lung on the microfluidic model. The traditional nude mouse model of lung metastasis was performed to investigate the physiological similarity of the microfluidic model to animal models. It was found that the metastatic potential of different cancer cells assessed by the microfluidic model was in agreement with that assessed by the nude mouse model. Furthermore, it was demonstrated that the metastatic inhibitor AMD3100 inhibited lung metastasis effectively in both the microfluidic model and the nude mouse model. Then the microfluidic model was used to mimick liver and bone metastasis of CTCs and confirm the potential for research of multiple-organ metastasis. Thus, the metastasis of CTCs to different organs was reconstituted on the microfluidic model. It may expand the capabilities of traditional cell culture models, providing a low-cost, time-saving, and rapid alternative to animal models.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Oncology ; Cell Biology
关键词[WOS]	BREAST-CANCER METASTASIS ; ENDOTHELIAL BARRIER FUNCTION ; ANIMAL-MODELS ; IN-VIVO ; CHIP ; CULTURE ; FLOW ; PROGRESSION ; MIGRATION ; PATTERNS
收录类别	SCI
语种	英语
WOS记录号	WOS:000389636000020
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/150428]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Dalian Med Univ, Coll Stomatol, Dalian, Peoples R China 2.Dalian Univ Technol, Fac Chem Environm & Biol Sci & Technol, Dalian, Peoples R China 3.Dalian Med Univ, Liaoning Prov Core Lab Glycobiol & Glycoengn, Dept Biochem & Mol Biol, Dalian, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Liu, Tingjiao,Kong, Jing,Luo, Yong,et al. A novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells[J]. ONCOTARGET,2016,7(48):78421-78432.
APA	Liu, Tingjiao.,Kong, Jing.,Luo, Yong.,Jin, Dong.,An, Fan.,...&Lin, Bingcheng.(2016).A novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells.ONCOTARGET,7(48),78421-78432.
MLA	Liu, Tingjiao,et al."A novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells".ONCOTARGET 7.48(2016):78421-78432.