Au-cysteine modified macroporous adsorption resin: preparation and highly selective enrichment and identification of N-linked glycopeptides from the complex biological sample

	Au-cysteine modified macroporous adsorption resin: preparation and highly selective enrichment and identification of N-linked glycopeptides from the complex biological sample
	Zhang, Weibing 1; Sun, Xudong 1; Dong, Jing 2; Li, Jinan 2; Ye, Mingliang 2; Zhang, Lingyi 1; Ou, Junjie 2
刊名	RSC ADVANCES
	2016
卷号	6 期号:114 页码:113058-113065
英文摘要	The development of hydrophilic stationary phase for highly efficient and selective enrichment and identification of low-abundance glycopeptides from a complex biological sample is an important prerequisite in glycoproteomics research. Herein, a hydrophilic cysteine functionalized macroporous adsorption resin/gold nanoparticle (MAR/Au-Cys) was synthesized and applied to the highly selective enrichment and identification of N-linked glycopeptides. The unique porous structure and high specific surface area of MAR provide abundant bonding sites for the immobilization of Au nanoparticles, and subsequently for the loading of cysteine via robust Au-S bonds. The structure and chemical composition of MAR/Au-Cys were confirmed by scanning electron microscopy, nitrogen adsorption-desorption analysis, mercury intrusion porosimetry and Fourier-transform infrared spectroscopy. Besides, the MAR/Au-Cys exhibited good performance in glycopeptide enrichment of tryptic digests from human immunoglobulin G and horseradish peroxidase by operation of a HILIC-SPE tip, revealing good universality, high enrichment selectivity, low detection limit (2.5 fmol) and satisfactory enrichment recovery (above 79%). Furthermore, the proposed material was successfully utilized in the N-glycoproteomics analysis of a complex biological sample. 1305 unique glycopeptides with 883 N-glycosylation sites from 476 different N-linked glycoproteins were identified from the proteins extracted from mouse liver. These results show that the proposed Au-cysteine modified macroporous adsorption resin is a promising affinity material for glycoproteomics research of real complex biological samples.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Multidisciplinary
关键词[WOS]	HYDROPHILIC INTERACTION CHROMATOGRAPHY ; FUNCTIONALIZED MAGNETIC NANOPARTICLES ; MASS-SPECTROMETRY ; EFFICIENT ENRICHMENT ; GLYCOPROTEINS ; GLYCOSYLATION ; SEPARATION ; HETEROGENEITY ; PURIFICATION ; REBAUDIOSIDE
收录类别	SCI
语种	英语
WOS记录号	WOS:000389905300039
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/150426]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Weibing,Sun, Xudong,Dong, Jing,et al. Au-cysteine modified macroporous adsorption resin: preparation and highly selective enrichment and identification of N-linked glycopeptides from the complex biological sample[J]. RSC ADVANCES,2016,6(114):113058-113065.
APA	Zhang, Weibing.,Sun, Xudong.,Dong, Jing.,Li, Jinan.,Ye, Mingliang.,...&Ou, Junjie.(2016).Au-cysteine modified macroporous adsorption resin: preparation and highly selective enrichment and identification of N-linked glycopeptides from the complex biological sample.RSC ADVANCES,6(114),113058-113065.
MLA	Zhang, Weibing,et al."Au-cysteine modified macroporous adsorption resin: preparation and highly selective enrichment and identification of N-linked glycopeptides from the complex biological sample".RSC ADVANCES 6.114(2016):113058-113065.