An ultrasensitive and conformation sensitive fluorescent probe for sensing human albumin in complex biological samples

	An ultrasensitive and conformation sensitive fluorescent probe for sensing human albumin in complex biological samples
	Ge, Guang-Bo 1,3; Cui, Jing-Nan 3; Wang, Yi-Ru 1,2; Feng, Lei 2,3; Xu, Liang 4; Hou, Jie 2,3; Jin, Qiang 1; Zhou, Nan 2; Lin, Yuan 2
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2017-06-01
卷号	245 页码:923-931
关键词	Human albumin (HA) Fluorescent probe Ultrahigh sensitivity Conformation sensitive Ligand-binding sites
英文摘要	In this study, an ultrasensitive and conformation sensitive fluorescent probe HMM (2-((6hydroxynaphthalen-2-yl) methylene) malononitrile) was developed for highly selective detection of human albumin (HA) in complex biological samples. Under physiological conditions, HMM could selectively bind on HA and form a complex which exhibited strong fluorescence signal around 562 nm. Both Job's Plot analysis and displacement assays using a series of site markers of HA demonstrated that HMM could simultaneously bind on two major ligand-binding sites (site I and site II) of HA, which partially explained why HMM could trigger more than 300-fold enhancement in fluorescence signal. The newly developed probe exhibited ultrahigh sensitivity (LOD =50 ngmL(-1)) and excellent selectivity toward HA over other human serum proteins. HMM has been successfully used to detect HA in diluted human plasma and urine, and the results demonstrate that such detection is highly reliable. Unlike bromocresol green (a commonly used dye-binding probe of HA), HMM is a conformation sensitive fluorescent probe for HA and it could distinguish between native and denatured HA. All these features demonstrated that HMM could serve as a practical and powerful tool for HA associated clinical routine tests including quantification of serum and urinary albumin. (C) 2017 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Physical Sciences ; Technology
类目[WOS]	Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
研究领域[WOS]	Chemistry ; Electrochemistry ; Instruments & Instrumentation
关键词[WOS]	HUMAN SERUM-ALBUMIN ; HUMAN CARBOXYLESTERASE 2 ; EMISSION CHARACTERISTICS ; SELECTIVE DETECTION ; BINDING ; PLASMA ; DRUG ; PROTEINS ; IDENTIFICATION ; NANOPARTICLES
收录类别	SCI
语种	英语
WOS记录号	WOS:000398760700108
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/150335]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China 2.Dalian Med Univ, Dalian 116044, Peoples R China 3.Dalian Univ Technol, State Key Lab Fine Chem, Dalian, Peoples R China 4.Dalian Univ Technol, Sch Chem, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Ge, Guang-Bo,Cui, Jing-Nan,Wang, Yi-Ru,et al. An ultrasensitive and conformation sensitive fluorescent probe for sensing human albumin in complex biological samples[J]. SENSORS AND ACTUATORS B-CHEMICAL,2017,245:923-931.
APA	Ge, Guang-Bo.,Cui, Jing-Nan.,Wang, Yi-Ru.,Feng, Lei.,Xu, Liang.,...&Lin, Yuan.(2017).An ultrasensitive and conformation sensitive fluorescent probe for sensing human albumin in complex biological samples.SENSORS AND ACTUATORS B-CHEMICAL,245,923-931.
MLA	Ge, Guang-Bo,et al."An ultrasensitive and conformation sensitive fluorescent probe for sensing human albumin in complex biological samples".SENSORS AND ACTUATORS B-CHEMICAL 245(2017):923-931.