Microchip-based single-cell functional proteomics for biomedical applications

	Microchip-based single-cell functional proteomics for biomedical applications
	Lu, Yao 1; Yang, Liu 2; Wei, Wei 3,4; Shi, Qihui 2
刊名	LAB ON A CHIP
	2017-04-07
卷号	17 期号:7 页码:1250-1263
英文摘要	Cellular heterogeneity has been widely recognized but only recently have single cell tools become available that allow characterizing heterogeneity at the genomic and proteomic levels. We review the technological advances in microchip-based toolkits for single-cell functional proteomics. Each of these tools has distinct advantages and limitations, and a few have advanced toward being applied to address biological or clinical problems that traditional population-based methods fail to address. High-throughput single-cell proteomic assays generate high-dimensional data sets that contain new information and thus require developing new analytical frameworks to extract new biology. In this review article, we highlight a few biological and clinical applications in which microchip-based single-cell proteomic tools provide unique advantages. The examples include resolving functional heterogeneity and dynamics of immune cells, dissecting cell-cell interaction by creating a well-controlled on-chip microenvironment, capturing high-resolution snapshots of immune system functions in patients for better immunotherapy and elucidating phosphoprotein signaling networks in cancer cells for guiding effective molecularly targeted therapies.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
关键词[WOS]	DROPLET-BASED MICROFLUIDICS ; MASS-SPECTROMETRY ; CANCER-CELLS ; FLOW-CYTOMETRY ; T-CELLS ; CYTOKINE SECRETION ; PROTEINS ; HETEROGENEITY ; DRUG ; EXPRESSION
收录类别	SCI
语种	英语
WOS记录号	WOS:000398955800004
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/150334]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China 2.Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Minist Educ, Key Lab Syst Biomed, Shanghai, Peoples R China 3.Univ Calif Los Angeles, David Geffen Sch Med, Dept Mol & Med Pharmacol, Los Angeles, CA 90095 USA 4.Univ Calif Los Angeles, David Geffen Sch Med, Jonsson Comprehens Canc Ctr, Los Angeles, CA 90095 USA
推荐引用方式 GB/T 7714	Lu, Yao,Yang, Liu,Wei, Wei,et al. Microchip-based single-cell functional proteomics for biomedical applications[J]. LAB ON A CHIP,2017,17(7):1250-1263.
APA	Lu, Yao,Yang, Liu,Wei, Wei,&Shi, Qihui.(2017).Microchip-based single-cell functional proteomics for biomedical applications.LAB ON A CHIP,17(7),1250-1263.
MLA	Lu, Yao,et al."Microchip-based single-cell functional proteomics for biomedical applications".LAB ON A CHIP 17.7(2017):1250-1263.