Exosome cofactor hMTR4 competes with export adaptor ALYREF to ensure balanced nuclear RNA pools for degradation and export

	Exosome cofactor hMTR4 competes with export adaptor ALYREF to ensure balanced nuclear RNA pools for degradation and export
	Fan, Jing 4; Kuai, Bin 4; Wu, Guifen 4; Wu, Xudong 2; Chi, Binkai 4; Wang, Lantian 4; Wang, Ke 4; Shi, Zhubing 4; Zhang, Heng 4; Chen, She 3
刊名	EMBO JOURNAL
	2017-10-02
卷号	36 期号:19 页码:2870-2886
关键词	ALYREF ARS2 hMTR4 mRNA export nuclear RNA degradation
英文摘要	The exosome is a key RNA machine that functions in the degradation of unwanted RNAs. Here, we found that significant fractions of precursors and mature forms of mRNAs and long noncoding RNAs are degraded by the nuclear exosome in normal human cells. Exosome-mediated degradation of these RNAs requires its cofactor hMTR4. Significantly, hMTR4 plays a key role in specifically recruiting the exosome to its targets. Furthermore, we provide several lines of evidence indicating that hMTR4 executes this role by directly competing with the mRNA export adaptor ALYREF for associating with ARS2, a component of the cap-binding complex (CBC), and this competition is critical for determining whether an RNA is degraded or exported to the cytoplasm. Together, our results indicate that the competition between hMTR4 and ALYREF determines exosome recruitment and functions in creating balanced nuclear RNA pools for degradation and export.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]	Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]	INTRONLESS MESSENGER-RNAS ; CAP-BINDING COMPLEX ; HUMAN TREX COMPLEX ; SACCHAROMYCES-CEREVISIAE ; POLY(A) POLYMERASE ; TARGETING COMPLEX ; MAMMALIAN-CELLS ; QUALITY-CONTROL ; II TRANSCRIPTS ; RIBOSOMAL-RNA
收录类别	SCI
语种	英语
WOS记录号	WOS:000412115800007
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/150170]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, CAS MPG Partner Inst Computat Biol, CAS Key Lab Computat Biol, Shanghai, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Mol Modeling & Design, State Key Lab Mol React Dynam, Dalian, Peoples R China 3.Natl Inst Biol Sci, Beijing, Peoples R China 4.Univ Chinese Acad Sci, Chinese Acad Sci, State Key Lab Mol Biol,Shanghai Inst Biochem & Ce, Shanghai Key Lab Mol Androl,CAS Ctr Excellence Mo, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Fan, Jing,Kuai, Bin,Wu, Guifen,et al. Exosome cofactor hMTR4 competes with export adaptor ALYREF to ensure balanced nuclear RNA pools for degradation and export[J]. EMBO JOURNAL,2017,36(19):2870-2886.
APA	Fan, Jing.,Kuai, Bin.,Wu, Guifen.,Wu, Xudong.,Chi, Binkai.,...&Cheng, Hong.(2017).Exosome cofactor hMTR4 competes with export adaptor ALYREF to ensure balanced nuclear RNA pools for degradation and export.EMBO JOURNAL,36(19),2870-2886.
MLA	Fan, Jing,et al."Exosome cofactor hMTR4 competes with export adaptor ALYREF to ensure balanced nuclear RNA pools for degradation and export".EMBO JOURNAL 36.19(2017):2870-2886.