A hollow fiber system for simple generation of human brain organoids

	A hollow fiber system for simple generation of human brain organoids
	Zhu, Yujuan 1,4,5; Wang, Li 4,5; Yin, Fangchao 1,4,5; Yu, Yue 1,4,5; Wang, Yaqing 1,4,5; Liu, Hui 4,5; Wang, Hui 1,4,5; Sun, Ning 3; Liu, Haitao 1,4,5; Qin, Jianhua 1,2,4,5
刊名	INTEGRATIVE BIOLOGY
	2017-09-01
卷号	9 期号:9 页码:774-781
英文摘要	3D organoids exhibit near-physiological morphogenesis and histology relying on the self-organization of human pluripotent stem cells (hPSCs), representing a new class of in vitro model for studying developmental biology and diseases. An engineered approach is highly desirable to generate sufficient organoids in a simple and efficient manner. Herein, we present a new strategy for the simple formation of massive human brain organoids from hiPSCs within a hollow fiber reactor system by combining fiber materials with the developmental biology principle. A thin and finely adjustable calcium alginate (CaA) core-shell fiber was constructed using a multilayer coaxial laminar flow microfluidic system. The meter-long hollow fibers enabled neural differentiation of hiPSCs and simple formation of abundant brain organoids in a 3D matrix. The generated brain organoids displayed essential features of human brain organogenesis, including polarized neuroepithelium, cell type heterogeneity and discrete brain regions, resembling the early brain development. This approach is simple and easy to operate, which allows for simplified formation of massive brain organoids, overcoming the tedious procedures in conventional methods. In particular, the facile and scalable characteristics of hollow fibers are compatible with real-time observation and monitoring, as well as flexible tissue manipulations for downstream biological analysis. It might also provide a new platform to advance stem cell-derived organoid models and their utility in biomedical applications.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Cell Biology
研究领域[WOS]	Cell Biology
关键词[WOS]	CEREBRAL ORGANOIDS ; STEM-CELLS ; MICROFIBERS ; DISEASE ; DYNAMICS ; MODEL ; CHIP
收录类别	SCI
语种	英语
WOS记录号	WOS:000411014600005
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/150137]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, Shanghai, Peoples R China 3.Fudan Univ, Sch Basic Med Sci, Dept Physiol & Pathophysiol, Shanghai, Peoples R China 4.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Beijing, Peoples R China 5.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Yujuan,Wang, Li,Yin, Fangchao,et al. A hollow fiber system for simple generation of human brain organoids[J]. INTEGRATIVE BIOLOGY,2017,9(9):774-781.
APA	Zhu, Yujuan.,Wang, Li.,Yin, Fangchao.,Yu, Yue.,Wang, Yaqing.,...&Qin, Jianhua.(2017).A hollow fiber system for simple generation of human brain organoids.INTEGRATIVE BIOLOGY,9(9),774-781.
MLA	Zhu, Yujuan,et al."A hollow fiber system for simple generation of human brain organoids".INTEGRATIVE BIOLOGY 9.9(2017):774-781.