High-throughput metabolic profiling based on small amount of hepatic cells

	High-throughput metabolic profiling based on small amount of hepatic cells
	Wang, Zhichao; Gao, Peng; Piao, Hailong; Xu, Guowang; Lu, Xin; Zhou, Lina; Yin, Peiyuan; Luo, Ping; Tang, Ling
刊名	ELECTROPHORESIS
	2017-09-01
卷号	38 期号:18 页码:2296-2303
关键词	A small amount of cells Amino acid metabolism High throughput Metabolic profiling
英文摘要	Common metabolomics platforms require about 10(6) cells, which has a limited throughput due to the time-consuming steps of cell culture and preparation. There is a demand for metabolic profiling methods to improve analytical throughput and detection sensitivity based on small amount of cells. In this study, we proposed a high-throughput scheme, integrating 96-well plate cell cultivation, in-situ cell pretreatment, and sensitive dansylation labeling coupled with LC-MS analysis of metabolites inside HepG2 cells (of the order of magnitude of 10(3) cells in each well). A simple and rapid cell pretreatment was performed showing good extraction efficiency and good precision (the RSDs smaller than 5%) for polar metabolites. The recovery in metabolite extraction evaluated with three isotope-labeled amino acids was from 89.7 to 106.3% at low, medium, and high concentrations. The suitability of the method was illustrated by exploring influences of different fatty acids on HepG2 cells.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]	EMBRYONIC STEM-CELLS ; FATTY-ACIDS ; DRUG DEVELOPMENT ; CANCER ; CULTURE ; CATABOLISM ; TOXICITY ; ONCOLOGY ; GLYCINE ; DEATH
收录类别	SCI
语种	英语
WOS记录号	WOS:000410758400007
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/149919]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Wang, Zhichao,Gao, Peng,Piao, Hailong,et al. High-throughput metabolic profiling based on small amount of hepatic cells[J]. ELECTROPHORESIS,2017,38(18):2296-2303.
APA	Wang, Zhichao.,Gao, Peng.,Piao, Hailong.,Xu, Guowang.,Lu, Xin.,...&Tang, Ling.(2017).High-throughput metabolic profiling based on small amount of hepatic cells.ELECTROPHORESIS,38(18),2296-2303.
MLA	Wang, Zhichao,et al."High-throughput metabolic profiling based on small amount of hepatic cells".ELECTROPHORESIS 38.18(2017):2296-2303.