Chloroplasts Isolation from Chlamydomonas reinhardtii under Nitrogen Stress

	Chloroplasts Isolation from Chlamydomonas reinhardtii under Nitrogen Stress
	Yang, Miao 1,3,4; Jiang, Jun-Peng 1,4; Xie, Xi 2; Chu, Ya-Dong 1; Fan, Yan 1,4; Cao, Xu-Peng 1; Xue, Song 1; Chi, Zhan-You 3
刊名	FRONTIERS IN PLANT SCIENCE
	2017-08-29
卷号	8
关键词	Chlamydomonas chloroplast isolation triacylglycerol nitrogen stress fatty acid biomarkers
英文摘要	Triacylglycerols are produced in abundance through chloroplast and endoplasmic reticulum pathways in some microalgae exposed to stress, though the relative contribution of either pathway remains elusive. Characterization of these pathways requires isolation of the organelles. In this study, an efficient and reproducible approach, including homogenous batch cultures of nitrogen-deprived algal cells in photobioreactors, gentle cell disruption using a simple custom-made disruptor with mechanical shear force, optimized differential centrifugation and Percoll density gradient centrifugation, was developed to isolate chloroplasts from Chlamydomonas reinhardtii subjected to nitrogen stress. Using this approach, the maximum limited stress duration was 4 h and the stressed cells exhibited 19 and 32% decreases in intracellular chlorophyll and nitrogen content, respectively. Chloroplasts with 48 - 300 mu g chlorophyll were successfully isolated from stressed cells containing 10 mg chlorophyll. These stressed chloroplasts appeared intact, as monitored by ultrastructure observation and a novel quality control method involving the fatty acid biomarkers. This approach can provide sufficient quantities of intact stressed chloroplasts for subcellular biochemical studies in microalgae.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Plant Sciences
研究领域[WOS]	Plant Sciences
关键词[WOS]	INTACT CHLOROPLASTS ; INORGANIC CARBON ; HEAT-STRESS ; LOW CO2 ; CELLS ; BIOSYNTHESIS ; LIPIDS ; PHOTOSYNTHESIS ; FRACTIONATION ; ACCUMULATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000408660800001
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/149874]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioengn Grp, Dalian, Peoples R China 2.Liaoning Ocean & Fisheries Sci Res Inst, Dalian, Peoples R China 3.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Yang, Miao,Jiang, Jun-Peng,Xie, Xi,et al. Chloroplasts Isolation from Chlamydomonas reinhardtii under Nitrogen Stress[J]. FRONTIERS IN PLANT SCIENCE,2017,8.
APA	Yang, Miao.,Jiang, Jun-Peng.,Xie, Xi.,Chu, Ya-Dong.,Fan, Yan.,...&Chi, Zhan-You.(2017).Chloroplasts Isolation from Chlamydomonas reinhardtii under Nitrogen Stress.FRONTIERS IN PLANT SCIENCE,8.
MLA	Yang, Miao,et al."Chloroplasts Isolation from Chlamydomonas reinhardtii under Nitrogen Stress".FRONTIERS IN PLANT SCIENCE 8(2017).