Hydrogen bond based smart polymer for highly selective and tunable capture of multiply phosphorylated peptides

	Hydrogen bond based smart polymer for highly selective and tunable capture of multiply phosphorylated peptides
	Qing, Guangyan 3; Lu, Qi 3; Li, Xiuling 1; Liu, Jing 1; Ye, Mingliang 1; Liang, Xinmiao 1; Sun, Taolei 2,3
刊名	NATURE COMMUNICATIONS
	2017-09-06
卷号	8
英文摘要	Multisite phosphorylation is an important and common mechanism for finely regulating protein functions and subsequent cellular responses. However, this study is largely restricted by the difficulty to capture low-abundance multiply phosphorylated peptides (MPPs) from complex biosamples owing to the limitation of enrichment materials and their interactions with phosphates. Here we show that smart polymer can serve as an ideal platform to resolve this challenge. Driven by specific but tunable hydrogen bonding interactions, the smart polymer displays differential complexation with MPPs, singly phosphorylated and non-modified peptides. Importantly, MPP binding can be modulated conveniently and precisely by solution conditions, resulting in highly controllable MPP adsorption on material surface. This facilitates excellent performance in MPP enrichment and separation from model proteins and real biosamples. High enrichment selectivity and coverage, extraordinary adsorption capacities and recovery towards MPPs, as well as high discovery rates of unique phosphorylation sites, suggest its great potential in phosphoproteomics studies.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	MULTISITE PHOSPHORYLATION ; AFFINITY-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; PHOSPHOPEPTIDE ENRICHMENT ; PROTEIN-PHOSPHORYLATION ; PHOSPHOPROTEOMICS ; IDENTIFICATION ; PROTEOMICS ; TYROSINE ; DISEASE
收录类别	SCI
语种	英语
WOS记录号	WOS:000409458000015
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/149866]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China 2.Wuhan Univ Technol, Sch Chem Chem Engn & Life Sci, 122 Luoshi Rd, Wuhan 430070, Hubei, Peoples R China 3.Wuhan Univ Technol, State Key Lab Adv Technol Mat Synth & Proc, 122 Luoshi Rd, Wuhan 430070, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Qing, Guangyan,Lu, Qi,Li, Xiuling,et al. Hydrogen bond based smart polymer for highly selective and tunable capture of multiply phosphorylated peptides[J]. NATURE COMMUNICATIONS,2017,8.
APA	Qing, Guangyan.,Lu, Qi.,Li, Xiuling.,Liu, Jing.,Ye, Mingliang.,...&Sun, Taolei.(2017).Hydrogen bond based smart polymer for highly selective and tunable capture of multiply phosphorylated peptides.NATURE COMMUNICATIONS,8.
MLA	Qing, Guangyan,et al."Hydrogen bond based smart polymer for highly selective and tunable capture of multiply phosphorylated peptides".NATURE COMMUNICATIONS 8(2017).