4 '-Hydroxywogonin suppresses lipopolysaccharide-induced inflammatory responses in RAW 264.7 macrophages and acute lung injury mice

	4 '-Hydroxywogonin suppresses lipopolysaccharide-induced inflammatory responses in RAW 264.7 macrophages and acute lung injury mice
	Sun, Lei 2; Fan, Chao 2; Wu, Le-Hao 2; Zhang, Gu-Fang 2; Xu, Fangfang 1; Zhang, Shuo 2; Zhang, Xiuli 1; Yu, Yang 2; Zhang, Yan 2; Ye, Richard D.2,3
刊名	PLOS ONE
	2017-08-08
卷号	12 期号:8
英文摘要	4'-Hydroxywogonin (4'-HW), a flavonoid, has been isolated from various plants and shown to inhibit NO production in macrophages. However, the molecular mechanisms and its in vivo activity have not been determined. Our study aimed to investigate the mechanisms underlying the anti-inflammatory effects of 4'-HW in vitro and in vivo. We showed that 4'-HW potently reduced the expression levels of COX-2 and iNOS as well as their products, prostaglandin E2 (PGE2) and nitric oxide (NO) respectively, in LPS-stimulated RAW 264.7 macrophages. 4'-HW also suppressed LPS-induced pro-inflammatory cytokines at mRNA and protein levels. Moreover, 4'-HW blocked the interaction of TAK1 and TAB1 in LPS-stimulated RAW 264.7 macrophages, resulting in an inhibition of the TAK1/IKK/NF-kappa B signaling pathway. Furthermore, 4'-HW also reduced the phosphorylation of MAPKs and PI3/Akt signaling pathways in LPS-stimulated RAW 264.7 macrophages. 4'-HW was also significantly decreased the intracellular reactive oxygen species (ROS) level. The effect of 4'-HW was confirmed in vivo. 4'-HW exhibited potent protective effect against LPS-induced ALI in mice. These findings indicate that 4'-HW suppresses the LPS-induced response in vitro and in vivo. It is likely that the inhibition of the TAK1/IKK/NF-kappa B, MAPKs and PI3/AKT signaling pathways contribute to the anti-inflammatory effects of 4'-HW. Our study suggests that 4'-HW may be an important functional constituent in the plants and has the potential value to be developed as a novel anti-inflammatory agent.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	NF-KAPPA-B ; ANTIINFLAMMATORY ACTIVITY ; PROTEIN-KINASES ; GENE-EXPRESSION ; P38 MAPK ; WOGONIN ; ACTIVATION ; PATHWAYS ; PHOSPHORYLATION ; ALPHA
收录类别	SCI
语种	英语
WOS记录号	WOS:000407144700004
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/149830]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Pharm, Shanghai, Peoples R China 3.Univ Macau, Inst Chinese Med Sci, Macau, Peoples R China
推荐引用方式 GB/T 7714	Sun, Lei,Fan, Chao,Wu, Le-Hao,et al. 4 '-Hydroxywogonin suppresses lipopolysaccharide-induced inflammatory responses in RAW 264.7 macrophages and acute lung injury mice[J]. PLOS ONE,2017,12(8).
APA	Sun, Lei.,Fan, Chao.,Wu, Le-Hao.,Zhang, Gu-Fang.,Xu, Fangfang.,...&Ye, Richard D..(2017).4 '-Hydroxywogonin suppresses lipopolysaccharide-induced inflammatory responses in RAW 264.7 macrophages and acute lung injury mice.PLOS ONE,12(8).
MLA	Sun, Lei,et al."4 '-Hydroxywogonin suppresses lipopolysaccharide-induced inflammatory responses in RAW 264.7 macrophages and acute lung injury mice".PLOS ONE 12.8(2017).