A highly sensitive fluorescent turn-on biosensor for glycoproteins based on boronic acid functional polymer capped Mn-doped ZnS quantum dots

	A highly sensitive fluorescent turn-on biosensor for glycoproteins based on boronic acid functional polymer capped Mn-doped ZnS quantum dots
	Chang, Lifang 1; Wu, Haocheng 1; He, Xiwen 1; Chen, Langxing 1,2; Zhang, Yukui 1,3
刊名	ANALYTICA CHIMICA ACTA
	2017-12-01
卷号	995 页码:91-98
关键词	Mn-doped ZnS quantum dots Fluorescent sensor Turn-on Boronate affinity Glycoproteins
英文摘要	A simple fluorescence turn-on sensor has been designed for the highly sensitive detection of glycoproteins on the basis of boronic acid functional polymer capped Mn-doped ZnS quantum dots (QDs@MPS@AAPBA). In the absence of glycoproteins, the fluorescence emission intensity of the QDs@MPS@AAPBA was relatively weaker due to the effective electronic transfer from the QDs to the boron moieties on its surface. While the glycoproteins were introduced into the system, an obvious fluorescence enhancement was observed. It was attributed to the boron moieties covalent binding gly-cans of the glycoproteins resulting in the electronic transfer process being inhibited. Under the optimal conditions, this fluorescent probe not only could be applied in a wide pH range of 5.0-9.0, but also the binding constants and detection limits of the QDs@MPS@AAPBA for horseradish peroxidase (HRP) and transferrin (TRF) were up to 7.23 x 10(6) M-1, 1.53 x 10(7) M-1 and 1.44 x 10(-10) M, 3.36 x 10(-10) M, respectively. Finally, this proposed method has also been utilized for the TRF determination in serum without any complicated pretreatment and the recovery was in the range of 95.7%-103.0%. As a result, it is promising for application on the glycoproteins detection in complex biological samples. (C) 2017 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	MOLECULARLY IMPRINTED POLYMER ; DRUG-DELIVERY ; IN-VIVO ; AFFINITY ; CELLS ; CAPILLARY ; RECEPTOR ; GLUCOSE ; SURFACE ; PROBE
收录类别	SCI
语种	英语
WOS记录号	WOS:000414540200009
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/149688]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Nankai Univ, Res Ctr Analyt Sci, Tianjin Key Lab Biosensing & Mol Recognit, Coll Chem,State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China 2.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
推荐引用方式 GB/T 7714	Chang, Lifang,Wu, Haocheng,He, Xiwen,et al. A highly sensitive fluorescent turn-on biosensor for glycoproteins based on boronic acid functional polymer capped Mn-doped ZnS quantum dots[J]. ANALYTICA CHIMICA ACTA,2017,995:91-98.
APA	Chang, Lifang,Wu, Haocheng,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2017).A highly sensitive fluorescent turn-on biosensor for glycoproteins based on boronic acid functional polymer capped Mn-doped ZnS quantum dots.ANALYTICA CHIMICA ACTA,995,91-98.
MLA	Chang, Lifang,et al."A highly sensitive fluorescent turn-on biosensor for glycoproteins based on boronic acid functional polymer capped Mn-doped ZnS quantum dots".ANALYTICA CHIMICA ACTA 995(2017):91-98.