A conserved repetitive DNA element located in the centromeres of chromosomes in Medicago genus
Yu, Feng1,2; Liu, Ruijuan1,2; Wang, Haiqing2; Dou, Quanwen2,3
刊名GENES & GENOMICS
2017-08-01
卷号39期号:8页码:903-911
关键词Medicago Centromere Fluorescence in situ hybridization Satellite repeat
英文摘要In most eukaryotic species, centromeres harbor large arrays of tandem repeated satellite DNA sequences. In this study, we report on the genomic distribution of a centromere satellite repeat "MtR3" in Medicago genus and three distantly related genera. Fluorescence in situ hybridization (FISH) results showed MtR3 repeats were detected in the centromere regions in M. truncatula, M. minima, M. edgeworthii, M. ruthenica, M. caerulea, M. sativa, and M. falcata (4x), but no signals were discovered in M. lupulina, M. polymorpha, and M. falcata (2x), Melilotus officinalis, Crotalaria medicaginea, and Trifolium repens. However, sequence analysis showed this MtR3 DNA had genomic distribution in all species and was highly conserved across the entire Medicago genus and three other genera. The conservation and widespread presence suggested MtR3 repeats may play important roles in centromeric function.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity
关键词[WOS]SATELLITE DNA ; TANDEM REPEATS ; EVOLUTION ; ORGANIZATION ; FABACEAE ; HETEROCHROMATIN ; KINETOCHORE ; LEGUMINOSAE ; SEQUENCES ; HISTORY
收录类别SCI
语种英语
WOS记录号WOS:000406154800009
内容类型期刊论文
源URL[http://ir.nwipb.ac.cn/handle/363003/7072]  
专题西北高原生物研究所_中国科学院西北高原生物研究所
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Qinghai, Peoples R China
3.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Crop Mol Breeding, Xining 810008, Qinghai, Peoples R China
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GB/T 7714
Yu, Feng,Liu, Ruijuan,Wang, Haiqing,et al. A conserved repetitive DNA element located in the centromeres of chromosomes in Medicago genus[J]. GENES & GENOMICS,2017,39(8):903-911.
APA Yu, Feng,Liu, Ruijuan,Wang, Haiqing,&Dou, Quanwen.(2017).A conserved repetitive DNA element located in the centromeres of chromosomes in Medicago genus.GENES & GENOMICS,39(8),903-911.
MLA Yu, Feng,et al."A conserved repetitive DNA element located in the centromeres of chromosomes in Medicago genus".GENES & GENOMICS 39.8(2017):903-911.
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