Kinetics of lung tissue factor expression and procoagulant activity in bleomycin induced acute lung injury.

doi:10.1186/s40169-015-0063-4

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	Kinetics of lung tissue factor expression and procoagulant activity in bleomycin induced acute lung injury.
	Ma, Li; Shaver, Ciara M; Grove, Brandon S; Mitchell, Daphne B; Wickersham, Nancy E; Carnahan, Robert H; Cooper, Tracy L; Brake, Brittany E; Ware, Lorraine B; Bastarache, Julie A
刊名	Clinical and translational medicine
	2015-12
卷号	4 期号:1 页码:63
ISSN号	2001-1326
DOI	10.1186/s40169-015-0063-4
文献子类	Article
英文摘要	BACKGROUND: Activation of coagulation by expression of tissue factor (TF) in the airspace is a hallmark of acute lung injury (ALI) but the timing of TF activation in relationship to increases in lung permeability and inflammation are unknown.METHODS: To test the hypothesis that TF is upregulated early in the course of acute bleomycin lung injury and precedes increased permeability and inflammation we studied the early course of bleomycin-induced ALI in mice. Mice were treated with 0.04U intratracheal bleomycin or vehicle control and bronchoalveolar lavage (BAL) and lung tissue were collected daily for 7days. Whole lung TF mRNA was determined by QT-PCR. TF protein was assessed by ELISA and immunostaining. BAL procoagulant activity was measured by BAL clot time and thrombin-antithrombin complexes. Inflammation was assessed by BAL cell count, differentials and CXCL1/KC concentration. Lung permeability was assessed by BAL protein and lung wet to dry weight ratio.RESULTS: Expression of CXCL1 occurred by day 1. BAL protein and lung wet-to-dry weight ratio increased significantly by day 3. TF mRNA and BAL procoagulant activity peaked on day 4 while whole lung TF protein peaked on day 6. Changes in permeability and procoagulant activity preceded inflammatory cell influx which was maximal at day 6 while whole lung TF protein peaked along with inflammation.CONCLUSION: These data demonstrate that cytokine upregulation is the earliest response to bleomycin administration, followed by increased lung permeability, upregulation of TF, and recruitment of inflammatory cells.
语种	英语
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/179407]
专题	第二临床医学院_期刊论文
推荐引用方式 GB/T 7714	Ma, Li,Shaver, Ciara M,Grove, Brandon S,et al. Kinetics of lung tissue factor expression and procoagulant activity in bleomycin induced acute lung injury.[J]. Clinical and translational medicine,2015,4(1):63.
APA	Ma, Li.,Shaver, Ciara M.,Grove, Brandon S.,Mitchell, Daphne B.,Wickersham, Nancy E.,...&Bastarache, Julie A.(2015).Kinetics of lung tissue factor expression and procoagulant activity in bleomycin induced acute lung injury..Clinical and translational medicine,4(1),63.
MLA	Ma, Li,et al."Kinetics of lung tissue factor expression and procoagulant activity in bleomycin induced acute lung injury.".Clinical and translational medicine 4.1(2015):63.