Standardized fluorescence in situ hybridization testing based on an appropriate panel of probes more effectively identifies common cytogenetic abnormalities in myelodysplastic syndromes than conventional cytogenetic analysis: A multicenter prospective study of 2302 patients in China

doi:10.1016/j.leukres.2015.02.005

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	Standardized fluorescence in situ hybridization testing based on an appropriate panel of probes more effectively identifies common cytogenetic abnormalities in myelodysplastic syndromes than conventional cytogenetic analysis: A multicenter prospective study of 2302 patients in China
	Lai, YY; Huang, XJ; Li, J; Zou, P; Xu, ZF; Sun, H; Shao, ZH; Zhou, DB; Chen, FP; Liu, ZG
刊名	LEUKEMIA RESEARCH
	2015-05
卷号	39 期号:5 页码:530-535
关键词	Myelodysplastic Syndrome Fluorescence In Situ Hybridization Cytogenetics
ISSN号	0145-2126
DOI	10.1016/j.leukres.2015.02.005
文献子类	Article
英文摘要	In an attempt to establish the advantages of fluorescence in situ hybridization (FISH) studies over conventional cytogenetic (CC) analysis, a total of 2302 de novo MDS patients from 31 Chinese institutions were prospectively selected in the present study for both CC and standardized FISH analysis for +8, -717q-, -515q-, 20q- and-Y chromosomal abnormalities. CC analysis was successful in 94.0% of the patients; of these patients, 35.9% of the cases were abnormal. FISH analysis was successful in all 2302 patients and detected at least one type of common cytogenetic abnormality in 42.7% of the cases. The incidences of +8, -717q-, -515q-, 20q- and-Y chromosomal abnormalities by FISH were 4.1% to 8.7% higher than those by CC. FISH identified abnormalities in 23.6% of the patients exhibiting normal CC results and revealed that 20.7% of the patients with adequate normal metaphases (>= 20) had abnormal clones. FISH identified cytogenetic abnormalities in 50.4% of the patients with failed CC analysis. In summary, our multicenter studies emphasised and confirmed the importance of applying standardized FISH testing based on an appropriate panel of probes to detect common cytogenetic abnormalities in Chinese de novo MDS patients, particularly those with normal or failed CC results. (C) 2015 Published by Elsevier Ltd.
学科主题	Oncology ; Hematology
项目编号	Chinese Medical Association on clinical application of molecular biology technique [CAMB042010]
语种	英语
WOS记录号	WOS:000352962100008
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/179354]
专题	第二临床医学院_期刊论文
通讯作者	Huang, XJ (reprint author), Peking Univ, Inst Hematol, Peoples Hosp, 11 Xizhimen South St, Beijing 100871, Peoples R China.
推荐引用方式 GB/T 7714	Lai, YY,Huang, XJ,Li, J,et al. Standardized fluorescence in situ hybridization testing based on an appropriate panel of probes more effectively identifies common cytogenetic abnormalities in myelodysplastic syndromes than conventional cytogenetic analysis: A multicenter prospective study of 2302 patients in China[J]. LEUKEMIA RESEARCH,2015,39(5):530-535.
APA	Lai, YY.,Huang, XJ.,Li, J.,Zou, P.,Xu, ZF.,...&Huang, XJ .(2015).Standardized fluorescence in situ hybridization testing based on an appropriate panel of probes more effectively identifies common cytogenetic abnormalities in myelodysplastic syndromes than conventional cytogenetic analysis: A multicenter prospective study of 2302 patients in China.LEUKEMIA RESEARCH,39(5),530-535.
MLA	Lai, YY,et al."Standardized fluorescence in situ hybridization testing based on an appropriate panel of probes more effectively identifies common cytogenetic abnormalities in myelodysplastic syndromes than conventional cytogenetic analysis: A multicenter prospective study of 2302 patients in China".LEUKEMIA RESEARCH 39.5(2015):530-535.