Distinct Expression Levels and Patterns of Stem Cell Marker, Aldehyde Dehydrogenase Isoform 1 (ALDH1), in Human Epithelial Cancers

doi:10.1371/journal.pone.0010277

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	Distinct Expression Levels and Patterns of Stem Cell Marker, Aldehyde Dehydrogenase Isoform 1 (ALDH1), in Human Epithelial Cancers
	Deng, S; Yang, XJ; Lassus, H; Liang, S; Kaur, S; Ye, QR; Li, CS; Wang, LP; Roby, KF; Orsulic, S
刊名	PLOS ONE
	2010-04-21
卷号	5 期号:4 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0010277
文献子类	Article
英文摘要	Aldehyde dehydrogenase isoform 1 (ALDH1) has been proved useful for the identification of cancer stem cells. However, our knowledge of the expression and activity of ALDH1 in common epithelial cancers and their corresponding normal tissues is still largely absent. Therefore, we characterized ALDH1 expression in 24 types of normal tissues and a large collection of epithelial tumor specimens (six cancer types, n = 792) by immunohistochemical staining. Using the ALDEFUOR assay, ALDH1 activity was also examined in 16 primary tumor specimens and 43 established epithelial cancer cell lines. In addition, an ovarian cancer transgenic mouse model and 7 murine ovarian cancer cell lines were analyzed. We found that the expression levels and patterns of ALDH1 in epithelial cancers are remarkably distinct, and they correlate with their corresponding normal tissues. ALDH1 protein expression levels are positively correlated with ALDH1 enzymatic activity measured by ALDEFLUOR assay. Long-term in vitro culture doesn't significantly affect ALDH1 activity in epithelial tumor cells. Consistent with research on other cancers, we found that high ALDH1 expression is significantly associated with poor clinical outcomes in serous ovarian cancer patients (n = 439, p = 0.0036). Finally, ALDH(br) tumor cells exhibit cancer stem cell properties and are resistant to chemotherapy. As a novel cancer stem cell marker, ALDH1 can be used for tumors whose corresponding normal tissues express ALDH1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer.
学科主题	Science & Technology - Other Topics
出版地	SAN FRANCISCO
项目编号	Breast Cancer Alliance ; Ovarian Cancer Research Found ; Mary Kay Ash Charitable Foundation ; National Cancer Institute [R01CA142776, P50-CA83638-7951] ; US Department of Defense [W81XWH-10-1-0082]
语种	英语
WOS记录号	WOS:000276952500022
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/126014]
专题	第二临床医学院_期刊论文
通讯作者	Deng, S (reprint author), Univ Penn, Ctr Res Early Detect & Cure Ovarian Canc, Philadelphia, PA 19104 USA.
推荐引用方式 GB/T 7714	Deng, S,Yang, XJ,Lassus, H,et al. Distinct Expression Levels and Patterns of Stem Cell Marker, Aldehyde Dehydrogenase Isoform 1 (ALDH1), in Human Epithelial Cancers[J]. PLOS ONE,2010,5(4):-.
APA	Deng, S.,Yang, XJ.,Lassus, H.,Liang, S.,Kaur, S.,...&Deng, S .(2010).Distinct Expression Levels and Patterns of Stem Cell Marker, Aldehyde Dehydrogenase Isoform 1 (ALDH1), in Human Epithelial Cancers.PLOS ONE,5(4),-.
MLA	Deng, S,et al."Distinct Expression Levels and Patterns of Stem Cell Marker, Aldehyde Dehydrogenase Isoform 1 (ALDH1), in Human Epithelial Cancers".PLOS ONE 5.4(2010):-.