Construction of Urothelium-Specific Recombinant Adenovirus and Its Inhibition in Bladder Cancer Cell

doi:10.1159/000200802

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	Construction of Urothelium-Specific Recombinant Adenovirus and Its Inhibition in Bladder Cancer Cell
	He, XD; Wang, ZP; Wei, HY; Zhou, Q; Wang, DG; Tian, JQ; Fu, SJ; Rodriguez, R; Wang, ZP (reprint author), Lanzhou Univ, Hosp 2, Inst Urol, Lanzhou 730030, Peoples R China.
刊名	UROLOGIA INTERNATIONALIS
	2009
卷号	82 期号:2 页码:209-213
关键词	Gene therapy Recombinant adenovirus Human uroplakin II Bladder cancer cell line
ISSN号	0042-1138
DOI	10.1159/000200802
文献子类	Article
英文摘要	Aim: To construct urothelium-specific recombinant adenovirus and investigate its inhibition in bladder cancer cell. Methods: RT-PCR analysis was used to determine expression patterns of hUPII and coxsackie adenovirus receptor on multiple cell lines. Transient transfection and luciferase detecting assay were used to detect tissue specificity of the hUPII promoter. Recombinant adenovirus Ad-UPII-E1A and AdUPII-Null were constructed. Restrictive enzyme digestion assay and PCR confirmed the correct construction. The adenovirus E1A protein expressed in BIU-87 was tested by Western blot after cells were infected with recombinant adenovirus. Recombinant adenovirus Ad-UPII-E1A was tested for its inhibition in bladder cancer cell line BIU-87. Results: HUPII and CAR were expressed and the hUPII promoter is highly active in bladder cancer cell line BIU-87. Using homologous recombination in bacteria technology, the hUPII promoter and E1A gene were inserted into the genome of type 5 recombinant adenovirus. The E1A protein was markedly positive in the samples of BIU-87 cells infected with recombinant adenovirus Ad-UPII-E1A. MTT assay demonstrated recombinant adenovirus Ad-UPII-E1A inhibited bladder cancer cell BIU-87 growth. Conclusion: The hUPII promoter shows high tissue specificity. Recombinant adenovirus Ad-UPII-E1A and AdUPII-Null were constructed and confirmed. Recombinant adenovirus Ad-UPII-E1A is effective in inhibition in bladder cancer cell line BIU-87. Copyright (C) 2009 S. Karger AG, Basel
学科主题	Urology & Nephrology
出版地	BASEL
语种	英语
WOS记录号	WOS:000264708900018
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/125999]
专题	第二临床医学院_期刊论文
通讯作者	Wang, ZP (reprint author), Lanzhou Univ, Hosp 2, Inst Urol, Lanzhou 730030, Peoples R China.
推荐引用方式 GB/T 7714	He, XD,Wang, ZP,Wei, HY,et al. Construction of Urothelium-Specific Recombinant Adenovirus and Its Inhibition in Bladder Cancer Cell[J]. UROLOGIA INTERNATIONALIS,2009,82(2):209-213.
APA	He, XD.,Wang, ZP.,Wei, HY.,Zhou, Q.,Wang, DG.,...&Wang, ZP .(2009).Construction of Urothelium-Specific Recombinant Adenovirus and Its Inhibition in Bladder Cancer Cell.UROLOGIA INTERNATIONALIS,82(2),209-213.
MLA	He, XD,et al."Construction of Urothelium-Specific Recombinant Adenovirus and Its Inhibition in Bladder Cancer Cell".UROLOGIA INTERNATIONALIS 82.2(2009):209-213.