Identification of Differentiation-Related Proteins in Gastric Adenocarcinoma Tissues by Proteomics

doi:10.1177/1533034615595792

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	Identification of Differentiation-Related Proteins in Gastric Adenocarcinoma Tissues by Proteomics
	Zhou, X; Yao, K; Zhang, L; Zhang, Y; Han, Y; Liu, HL; Liu, XW; Su, G; Yuan, WZ; Wei, XD
刊名	TECHNOLOGY IN CANCER RESEARCH & TREATMENT
	2016-10
卷号	15 期号:5 页码:697-706
关键词	gastric adenocarcinoma proteomics differentiation glycolysis immunohistochemistry Image-Pro Plus overall survival
ISSN号	1533-0346
DOI	10.1177/1533034615595792
文献子类	Article
英文摘要	There is a significant correlation between the degree of tumor differentiation and the survival of patients with gastric cancers. In this report, we compared proteomic differences between poorly differentiated gastric adenocarcinoma tissues and well-differentiated gastric adenocarcinoma tissues in order to identify differentiation-related proteins that may be closely correlated with differentiation of gastric cancer pathogenesis. We identified 7 proteins, of which calreticulin precursor, tapasinERP57 heterodimer, pyruvate kinase isozymes M1/M2 isoform M2, class Pi glutathione S-transferase, and chain A crystal structure of human enolase 1 were upregulated in poorly differentiated gastric adenocarcinoma compared with well-differentiated gastric adenocarcinoma, while myosin-11 isoform SM2A and actin alpha cardiac were downregulated. Two of them, pyruvate kinase isozymes M1/M2 isoform M2 and enolase 1 are enzymes involved in glycolytic pathway. The upregulation of pyruvate kinase isozymes M1/M2 isoform M2 and enolase 1 in poorly differentiated gastric adenocarcinoma was confirmed by Western blotting and immunohistochemistry. Furthermore, we observed 107 cases with gastric adenocarcinoma and found that the high expression of pyruvate kinase isozymes M1/M2 isoform M2 and enolase 1 correlates with tumor size (P = .0001 and P = .0017, respectively), depth of invasion (P = .0024 and P = .0261, respectively), and poor prognosis of patients. In conclusion, with this proteomic analysis, pyruvate kinase isozymes M1/M2 isoform M2 and enolase 1 were identified upregulated in poorly differentiated gastric adenocarcinoma comparing with well-differentiated gastric adenocarcinoma. The expression level of pyruvate kinase isozymes M1/M2 isoform M2 and enolase 1 was significantly correlated with overall survival. Some of them would be differentiation-related cancer biomarkers and are associated with tumor metastasis, invasion, and prognosis.
学科主题	Oncology
出版地	THOUSAND OAKS
资助项目	新世纪优秀人才支持计划 ; 长江学者和创新团队发展计划 ; 中央高校基本科研业务费专项资金
项目编号	Changjiang Scholar fund ; Ministry of Education of China [NCET-070403] ; Fundamental Research Funds for the Central Universities [lzujbky-2010-143]
语种	英语
WOS记录号	WOS:000383212400017
资助机构	MOE ; LZU
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/178672]
专题	第一临床医学院_期刊论文
通讯作者	Zhu, BD (reprint author), Lanzhou Univ, Inst Pathogen Biol, Sch Basic Med Sci, Lanzhou, Peoples R China.; Guan, QL (reprint author), Lanzhou Univ, Dept Surg Oncol, Affiliated Hosp 1, Lanzhou, Peoples R China.
推荐引用方式 GB/T 7714	Zhou, X,Yao, K,Zhang, L,et al. Identification of Differentiation-Related Proteins in Gastric Adenocarcinoma Tissues by Proteomics[J]. TECHNOLOGY IN CANCER RESEARCH & TREATMENT,2016,15(5):697-706.
APA	Zhou, X.,Yao, K.,Zhang, L.,Zhang, Y.,Han, Y.,...&Guan, QL .(2016).Identification of Differentiation-Related Proteins in Gastric Adenocarcinoma Tissues by Proteomics.TECHNOLOGY IN CANCER RESEARCH & TREATMENT,15(5),697-706.
MLA	Zhou, X,et al."Identification of Differentiation-Related Proteins in Gastric Adenocarcinoma Tissues by Proteomics".TECHNOLOGY IN CANCER RESEARCH & TREATMENT 15.5(2016):697-706.