Hepatitis B virus X protein (HBx)-induced abnormalities of nucleic acid metabolism revealed by H-1-NMR-based metabonomics

doi:10.1038/srep24430

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	Hepatitis B virus X protein (HBx)-induced abnormalities of nucleic acid metabolism revealed by H-1-NMR-based metabonomics
	Yue, D; Zhang, YW; Cheng, LL; Ma, JH; Xi, YF; Yang, LP; Su, C; Shao, B; Huang, AL; Xiang, R
刊名	SCIENTIFIC REPORTS
	2016-04-14
卷号	6
ISSN号	2045-2322
DOI	10.1038/srep24430
文献子类	Article
英文摘要	Hepatitis B virus X protein (HBx) plays an important role in HBV-related hepatocarcinogenesis; however, mechanisms underlying HBx-mediated carcinogenesis remain unclear. In this study, an NMR-based metabolomics approach was applied to systematically investigate the effects of HBx on cell metabolism. EdU incorporation assay was conducted to examine the effects of HBx on DNA synthesis, an important feature of nucleic acid metabolism. The results revealed that HBx disrupted metabolism of glucose, lipids, and amino acids, especially nucleic acids. To understand the potential mechanism of HBx-induced abnormalities of nucleic acid metabolism, gene expression profiles of HepG2 cells expressing HBx were investigated. The results showed that 29 genes involved in DNA damage and DNA repair were differentially expressed in HBx-expressing HepG2 cells. HBx-induced DNA damage was further demonstrated by karyotyping, comet assay, Western blotting, immunofluorescence and immunohistochemistry analyses. Many studies have previously reported that DNA damage can induce abnormalities of nucleic acid metabolism. Thus, our results implied that HBx initially induces DNA damage, and then disrupts nucleic acid metabolism, which in turn blocks DNA repair and induces the occurrence of hepatocellular carcinoma (HCC). These findings further contribute to our understanding of the occurrence of HCC.
学科主题	Science & Technology - Other Topics
出版地	LONDON
资助项目	国家自然科学基金项目 ; 国家科技重大专项
项目编号	National Natural Science Foundation of China [81101728] ; National Science and Technology Major Projects of New Drugs [2012ZX09103301-036] ; National Science and Technology Major Project for Infectious Diseases Control [2012ZX10002014-004, 2016ZX10002024-004] ; Science and Technology Support Program of Sichuan [2014SZ0122]
语种	英语
WOS记录号	WOS:000374058500001
资助机构	NSFC ; MOST
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/178641]
专题	第一临床医学院_期刊论文
通讯作者	Cheng, P (reprint author), Sichuan Univ, West China Hosp, State Key Lab Biotherapy, Chengdu 610041, Peoples R China.; Cheng, P (reprint author), Sichuan Univ, West China Hosp, Collaborat Innovat Ctr Biotherapy, Ctr Canc, Chengdu 610041, Peoples R China.
推荐引用方式 GB/T 7714	Yue, D,Zhang, YW,Cheng, LL,et al. Hepatitis B virus X protein (HBx)-induced abnormalities of nucleic acid metabolism revealed by H-1-NMR-based metabonomics[J]. SCIENTIFIC REPORTS,2016,6.
APA	Yue, D.,Zhang, YW.,Cheng, LL.,Ma, JH.,Xi, YF.,...&Cheng, P .(2016).Hepatitis B virus X protein (HBx)-induced abnormalities of nucleic acid metabolism revealed by H-1-NMR-based metabonomics.SCIENTIFIC REPORTS,6.
MLA	Yue, D,et al."Hepatitis B virus X protein (HBx)-induced abnormalities of nucleic acid metabolism revealed by H-1-NMR-based metabonomics".SCIENTIFIC REPORTS 6(2016).