Plasma Sphingolipids as Potential Indicators of Hepatic Necroinflammation in Patients with Chronic Hepatitis C and Normal Alanine Aminotransferase Level

doi:10.1371/journal.pone.0095095

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	Plasma Sphingolipids as Potential Indicators of Hepatic Necroinflammation in Patients with Chronic Hepatitis C and Normal Alanine Aminotransferase Level
	Li, JF; Qu, F; Zheng, SJ; Ren, JY; Wu, HL; Liu, M; Liu, H; Ren, F; Chen, Y; Zhang, JL
刊名	PLOS ONE
	2014-04-15
卷号	9 期号:4 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0095095
文献子类	Article
英文摘要	Accurate estimation of hepatic necroinflammation caused by chronic hepatitis C (CHC) is crucial for prediction of prognosis and design of therapeutic strategy, which is particularly true for CHC patients with normal alanine aminotransferase (ALT) level. Recent studies have shown that sphingolipids have a close relationship with hepatitis C virus infection. The present study aimed to identify plasma sphingolipids related to hepatic necroinflammation. We included 120 treatment-naive CHC patients and 64/120 had normal ALT levels (<40 U/L). CHC patients who underwent liver biopsies were subjected to Scheuer scoring analysis for scope of hepatic inflammation. Plasma sphingolipids were detected by high-performance liquid chromatography tandem mass spectrometry. Our results showed 44 plasma sphingolipids were detected altogether. Of all detected sphingolipids, hexosylceramide (HexCer) (d18:1/22:0) and HexCer (d18:1/24:0) showed a significant difference among G0/G1, G2, and G3/G4 (P<0.05). For identifying hepatic necroinflammation (G >= 2), after adjusting other factors, the odds ratio (OR) of HexCer (d18:1/22:0) reached 1.01 (95% confidence interval [CI]:1.00-1.02). Furthermore, the area under the curve (AUC) of HexCer (d18:1/22:0) was 0.7 (P = 0.01) and approached that of ALT (AUC = 0.78). However, in CHC patients with normal ALT, HexCer (d18:1/22:0) was an independent factor (OR:1.02, 95% CI:1.01-1.03) to identify the hepatic necroinflammation (G >= 2). HexCer (d18:1/22:0) not only showed the largest AUC (0.78, P = 0.001), but also exhibited the highest specificity of all indicators. These results indicate that plasma HexCer (d18:1/22:0) is a potential indicator to distinguish hepatic necroinflammation in CHC patients. For CHC with normal ALT, the ability of HexCer (d18:1/22:0) to distinguish hepatic necroinflammation might be superior to conventional serum indicators.
学科主题	Science & Technology - Other Topics
出版地	SAN FRANCISCO
资助项目	国家科技重大专项
项目编号	National Science and Technology Key Project on "Major Infectious Diseases such as HIV/AIDS, Viral Hepatitis Prevention and Treatment" [2012ZX10002004-006, 2012ZX10004904-003-001, 2013ZX10002002-006] ; Ministry of Science and Technology of People's Republic of China [2012ZX09301002-006] ; High Technical Personnel Training Item in Beijing Health System [2011-3-083] ; Beijing Municipal Science & Technology Commission [Z131107002213019] ; Special Scientific Research Fund for Beijing Health Development [2011-2018-04] ; YouAn Scientific Research Fund for Liver Disease and HIV/AIDS [BJYAH-2011-045]
语种	英语
WOS记录号	WOS:000336922600145
资助机构	MOST
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/125899]
专题	第一临床医学院_期刊论文
通讯作者	Zhang, JL (reprint author), Chinese Acad Med Sci, Inst Mat Med, State Key Lab Bioact Subst & Funct Nat Med, Beijing 100730, Peoples R China.
推荐引用方式 GB/T 7714	Li, JF,Qu, F,Zheng, SJ,et al. Plasma Sphingolipids as Potential Indicators of Hepatic Necroinflammation in Patients with Chronic Hepatitis C and Normal Alanine Aminotransferase Level[J]. PLOS ONE,2014,9(4):-.
APA	Li, JF.,Qu, F.,Zheng, SJ.,Ren, JY.,Wu, HL.,...&Zhang, JL .(2014).Plasma Sphingolipids as Potential Indicators of Hepatic Necroinflammation in Patients with Chronic Hepatitis C and Normal Alanine Aminotransferase Level.PLOS ONE,9(4),-.
MLA	Li, JF,et al."Plasma Sphingolipids as Potential Indicators of Hepatic Necroinflammation in Patients with Chronic Hepatitis C and Normal Alanine Aminotransferase Level".PLOS ONE 9.4(2014):-.