Interaction between cyclooxygenase-2, Snail, and E-cadherin in gastric cancer cells

doi:10.3748/wjg.v19.i37.6265

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	Interaction between cyclooxygenase-2, Snail, and E-cadherin in gastric cancer cells
	Liu, XJ; Chen, ZF; Li, HL; Hu, ZN; Liu, M; Tian, AP; Zhao, D; Wu, J; Zhou, YN; Qiao, L
刊名	WORLD JOURNAL OF GASTROENTEROLOGY
	2013-10-07
卷号	19 期号:37 页码:6265-6271
关键词	Cyclooxygenase-2 E-cadherin celecoxib Prostaglandin E2 Gastric cancer
ISSN号	1007-9327
DOI	10.3748/wjg.v19.i37.6265
其他题名	Interaction between cyclooxygenase-2;Snail;and E-cadherin in gastric cancer cells
文献子类	Article
英文摘要	AIM: To investigate the mechanisms of how cyclooxygenase-2 (COX-2) regulates E-cadherin in gastric cancer cells. METHODS: COX-2 expression in human gastric cancer cell lines SGC-7901, BGC-823, MGC-803 and AGS were measured at the mRNA and protein level. COX-2 rich cell line SGC-7901 was chosen for subsequent experiments. siRNA mediated gene knockdown was used to investigate the impact of COX-2 on nuclear factor-kappa B NF-kappa B), Snail, and E-cadherin in gastric cancer cells. Gene expression was determined by Western blot and real-time polymerase chain reaction. To analyze whether NF-.B inhibition could interrupt the modulatory effect of COX-2 or prostaglandin E2 (PGE2) on E-cadherin, gastric cancer cells were treated with celecoxib or PGE2, in the presence of NF-.B specific siRNA. RESULTS: Highest expression level of COX-2 was found in SGC-7901 cells, both at mRNA and protein levels. siRNA mediated down-regulation of COX-2 led to a reduced expression of NF-.B and Snail, but an increased expression of E-cadherin in SGC-7901 cells. siRNA mediated down-regulation of NF-.B also led to a reduced expression of E-cadherin and Snail in SGC-7901 cells. However, COX-2 expression did not alter after cells were treated with NF-kappa B specific siRNA in SGC-7901 cells. Treatment of SGC-7901 cells with celecoxib led to a reduced expression of Snail but an increased expression of E-cadherin. In contrast, treatment of SGC-7901 cells with PGE2 led to an increased Snail and a decreased E-cadherin. However, siRNAmediated knockdown of NF-kappa B partially abolished the effect of celecoxib and PGE2 on the regulation of E-cadherin and Snail in SGC-7901 cells. CONCLUSION: COX-2 likely functions upstream of NF kappa B and regulates the expression of E-cadherin via NF kappa B/Snail signaling pathway in gastric cancer cells. (C) 2013 Baishideng. All rights reserved.
学科主题	Gastroenterology & Hepatology
出版地	WANCHAI
资助项目	中央高校基本科研业务费专项资金 ; 甘肃省技术研究与开发专项计划
项目编号	National Natural Science Funding of China [81172366] ; Fundamental Research Funds for the Central Universities [lzujbky-2012-224 ; ] ; Gansu Special Program for High Technology Research and Development [0912TCYA027]
语种	英语
WOS记录号	WOS:000324909900018
资助机构	LZU ; GSSTD
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/125807]
专题	第一临床医学院_期刊论文
通讯作者	Qiao, L (reprint author), Westmead Hosp, Fac Med, Western Clin Sch, Storr Liver Unit,Westmead Millennium Inst, Westmead, NSW 2145, Australia.
推荐引用方式 GB/T 7714	Liu, XJ,Chen, ZF,Li, HL,et al. Interaction between cyclooxygenase-2, Snail, and E-cadherin in gastric cancer cells[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2013,19(37):6265-6271.
APA	Liu, XJ.,Chen, ZF.,Li, HL.,Hu, ZN.,Liu, M.,...&Qiao, L .(2013).Interaction between cyclooxygenase-2, Snail, and E-cadherin in gastric cancer cells.WORLD JOURNAL OF GASTROENTEROLOGY,19(37),6265-6271.
MLA	Liu, XJ,et al."Interaction between cyclooxygenase-2, Snail, and E-cadherin in gastric cancer cells".WORLD JOURNAL OF GASTROENTEROLOGY 19.37(2013):6265-6271.