In vitro cytotoxic studies of ultrafine TiO2/graphene oxide sheet nanocomposites in human alveolar epithelial cells

doi:10.4028/www.scientific.net/AMR.1051.353

CORC > 兰州大学 > 兰州大学 > 口腔医学院 > 会议论文

	In vitro cytotoxic studies of ultrafine TiO2/graphene oxide sheet nanocomposites in human alveolar epithelial cells
	Gao, Jing; Yuan, Xue-Shun; Wang, Yu; Cao, Lin; Dong, Peng-Yu; Cao, Bao-Cheng
	2014
会议日期	September 21, 2014 - September 22, 2014
会议地点	Hong Kong, China
关键词	X ray photoelectron spectroscopy Biocompatibility Cell death Cells Cytology Cytotoxicity Graphene Nanocomposites Nanoparticles Photoelectron spectroscopy Sustainable development Titanium dioxide Transmission electron microscopy A549 cells Alveolar epithelial cells Brunauer-Emmett-Teller method Cell viability Energy dispersive x-ray LDH Methyl thiazolyl tetrazolium assays TiO
卷号	1051
DOI	10.4028/www.scientific.net/AMR.1051.353
页码	353-362
英文摘要	The novel TiO2/graphene sheet (TiO2/GSs) nanocomposites were synthesized using graphene oxide sheets and TiCl3. All target composites were characterized by transmission electron microscopy (TEM), X-ray electron diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and energy dispersive X-ray spectra (EDX), and the surface areas were tested with the Brunauer-Emmett-Teller (BET) method. We studied the cytotoxicity of TiO2/GSs nanoparticles on A549 cells by examining the influence of TiO2/GSs on cell morphology and viability by methyl thiazolyl tetrazolium (MTT) assay. We also determined membrane integrity and apoptosis of A549 cells after different doses of TiO2/GSs exposure by LDH assay and flow cytometry. Assay of A549 cell viability showed regular reductions with a time- and dose-dependent tendency after exposure to pure TiO2, TiO2/4.2wt% GSs and TiO2/6wt% GSs for 24 hrs or 48 hrs. The LDH released and cellular apoptosis also had a dose-dependent effect, which was associated with the surface area of TiO2/4.2wt% GSs. Our results provide essential knowledge of the acceptable biocompatibility of TiO2/GSs nanocomposites, and only when cells were exposed at a high concentration (≥50 μg/mL), and for a prolonged period of time did TiO2/GSs nanoparticles exhibit minimal cytotoxicity against A549 cells. © (2014) Trans Tech Publications, Switzerland.
会议录	Advanced Materials Research
学科主题	Electric And Magnetic Materials ; Optical Devices And Systems ; Nanotechnology ; Chemistry ; Chemical Products Generally ; Inorganic Compounds ; Industrial Economics
语种	英语
ISSN号	1022-6680
内容类型	会议论文
源URL	[http://ir.lzu.edu.cn/handle/262010/185271]
专题	口腔医学院_会议论文
通讯作者	Cao, Bao-Cheng
推荐引用方式 GB/T 7714	Gao, Jing,Yuan, Xue-Shun,Wang, Yu,et al. In vitro cytotoxic studies of ultrafine TiO2/graphene oxide sheet nanocomposites in human alveolar epithelial cells[C]. 见:. Hong Kong, China. September 21, 2014 - September 22, 2014.