Slit2-Robo1 signaling promotes the adhesion, invasion and migration of tongue carcinoma cells via upregulating matrix metalloproteinases 2 and 9, and downregulating E-cadherin

doi:10.3892/mmr.2016.5518

CORC > 兰州大学 > 兰州大学 > 口腔医学院 > 期刊论文

	Slit2-Robo1 signaling promotes the adhesion, invasion and migration of tongue carcinoma cells via upregulating matrix metalloproteinases 2 and 9, and downregulating E-cadherin
	Zhao, Y; Zhou, FL; Li, WP; Wang, J; Wang, LJ; Wang, LJ (reprint author), Guangdong Pharmaceut Univ, Vasc Biol Res Inst, 280 Wai Huan Dong Rd, Guangzhou 510006, Guangdong, Peoples R China.
刊名	MOLECULAR MEDICINE REPORTS
	2016-09
卷号	14 期号:3 页码:1901-1906
关键词	adhesion cancer research metastasis tumor cell culture tumor markers
ISSN号	1791-2997
DOI	10.3892/mmr.2016.5518
文献子类	Article
英文摘要	Whether Slit homologue 2 (Slit2) inhibits or promotes tumor cell migration remains controversial, and the role of Slit2-Roundabout 1 (Robo1) signaling in oral cancer remains to be fully elucidated. The aim of the present study was to investigate the role of Slit2-Robo1 signaling in the adhesion, invasion and migration of tongue carcinoma cells, and the mechanism by which Slit2-Robo1 signaling inhibits or promotes tumor cell migration. Tca8113 tongue carcinoma cells were treated with the monoclonal anti-human Robo1 antibody, R5, to inhibit the Slit2-Robo1 signaling pathway, with immunoglobulin (Ig)G2b treatment as a negative control. The expression levels of Slit2 and Robo1 were determined using flow cytometry. The effects of R5 on the adhesion, invasion and migration of Tca8113 tongue carcinoma cells were investigated. Gelatin zymography was used to investigate the activity of matrix metalloproteinase 2 (MMP2) and MMP9. Western blot analysis was used to evaluate the expression levels of E-cadherin in Tca8113 cells treated with 10 mu g/ml of either R5 or IgG2b. Slit2 and Robo1 proteins were found to be expressed in the Tca8113 cells. R5 significantly inhibited the adhesion, invasion and migration of Tca8113 cells in vitro. R5 also inhibited the activities of MMP2 and MMP9, and increased the expression of E-cadherin in the Tca8113 cells. These results suggested that Slit2-Robo1 signaling promoted the adhesion, invasion and migration of tongue carcinoma cells by upregulating the expression levels of MMP2 and MMP9 and, downregulating the expression of E-cadherin.
学科主题	Oncology ; Research & Experimental Medicine
出版地	ATHENS
资助项目	国家自然科学基金项目 ; 中央高校基本科研业务费专项资金 ; 甘肃省青年科技基金计划
项目编号	Fundamental Research Funds for the Central Universities, China [1007RJYA009] ; LanZhou University Ji Ben Ke Yan [lzujbky-2015-36, lzujbky-2015-290] ; National Natural Science Foundation of China [81500835]
语种	英语
WOS记录号	WOS:000382445400004
资助机构	NSFC ; LZU ; GSSTD
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/179507]
专题	口腔医学院_期刊论文
通讯作者	Wang, LJ (reprint author), Guangdong Pharmaceut Univ, Vasc Biol Res Inst, 280 Wai Huan Dong Rd, Guangzhou 510006, Guangdong, Peoples R China.
推荐引用方式 GB/T 7714	Zhao, Y,Zhou, FL,Li, WP,et al. Slit2-Robo1 signaling promotes the adhesion, invasion and migration of tongue carcinoma cells via upregulating matrix metalloproteinases 2 and 9, and downregulating E-cadherin[J]. MOLECULAR MEDICINE REPORTS,2016,14(3):1901-1906.
APA	Zhao, Y,Zhou, FL,Li, WP,Wang, J,Wang, LJ,&Wang, LJ .(2016).Slit2-Robo1 signaling promotes the adhesion, invasion and migration of tongue carcinoma cells via upregulating matrix metalloproteinases 2 and 9, and downregulating E-cadherin.MOLECULAR MEDICINE REPORTS,14(3),1901-1906.
MLA	Zhao, Y,et al."Slit2-Robo1 signaling promotes the adhesion, invasion and migration of tongue carcinoma cells via upregulating matrix metalloproteinases 2 and 9, and downregulating E-cadherin".MOLECULAR MEDICINE REPORTS 14.3(2016):1901-1906.