Investigate electrochemical immunosensor of cortisol based on gold nanoparticles/magnetic functionalized reduced graphene oxide

doi:10.1016/j.bios.2016.07.047

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	Investigate electrochemical immunosensor of cortisol based on gold nanoparticles/magnetic functionalized reduced graphene oxide
	Sun, BL; Gou, YQ; Ma, YL; Zheng, XP; Bai, RB; Abdelmoaty, AAA; Hu, FD; Hu, FD (reprint author), Lanzhou Univ, Sch Pharm, Lanzhou 730000, Peoples R China.
	2017-02-15
会议日期	MAY 24-28, 2016
会议地点	Gothenburg, SWEDEN
关键词	Magnetic functionalized reduced graphene oxide (MrGO) Gold nanoparticles (AuNPs) Electrochemical immunosensor Cortisol (Cor)
卷号	88
DOI	10.1016/j.bios.2016.07.047
页码	55-62
英文摘要	A sensitively competitive electrochemical immunosensor for the detection of cortisol was successfully developed based on gold nanoparticles and magnetic functionalized reduced graphene oxide (AuNPs/ MrGO). In order to construct the base of the immunosensor, the MrGO was initially fabricated by chemical cross -linking and used to modify the nafion pretreated glassy carbon electrode. Subsequently, the surface of electrode was modified by AuNPs via electrochemical deposition. A variety of cortisol (Cor) can be firmly loaded in the AuNPs/MrGO with large specific surface area and good bioactivity to construct the basic electrode (Cor/AuNPs/MrGO/Nafion@GCE), which was characterized by the cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), respectively. Due to the cortisol on the surface of basic electrode and samples can competitively combine with the cortisol antibody labelled by horseradish peroxidase (HRP-Strept-Biotin-Ab). Finally, the detection signal of electrochemical immunosensor (HRP-Strept-Biotin-Ab-Cor/AuNPs/MrGO/Nafion@GCE) in the test liquid had negative correlations with the concentration of cortisol in samples. The AuNPs/MrGO with excellent electrical conductivity being applied, the electrochemical response of the immunosensor was immensely amplified. The immunosensor displayed excellent analytical performance for the detection of cortisol range from 0.1 to 1000 ng/mL with a detection limit of 0.05 ng/mL at 3 sigma. Moreover, compared the developed immunoassay with commercially available enzyme linked immunosorbent assay, the proposed method showed good precision, acceptable stability and reproducibility, indicating the immunosensor could be used for the sensitive, efficient and real-time detection of cortisol in real samples. Therefore, the present strategy provides a novel and convenient method for clinical determination of cortisol. (C) 2016 Elsevier B.V. All rights reserved.
会议录	BIOSENSORS & BIOELECTRONICS
会议录出版地	OXFORD
学科主题	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
语种	英语
ISSN号	0956-5663
WOS记录号	WOS:000389112700009
内容类型	会议论文
源URL	[http://ir.lzu.edu.cn/handle/262010/189608]
专题	药学院_会议论文
通讯作者	Hu, FD (reprint author), Lanzhou Univ, Sch Pharm, Lanzhou 730000, Peoples R China.
推荐引用方式 GB/T 7714	Sun, BL,Gou, YQ,Ma, YL,et al. Investigate electrochemical immunosensor of cortisol based on gold nanoparticles/magnetic functionalized reduced graphene oxide[C]. 见:. Gothenburg, SWEDEN. MAY 24-28, 2016.