Anti-proliferative activity and cell cycle arrest induced by evodiamine on paclitaxel-sensitive and -resistant human ovarian cancer cells

doi:10.1038/srep16415

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	Anti-proliferative activity and cell cycle arrest induced by evodiamine on paclitaxel-sensitive and -resistant human ovarian cancer cells
	Zhong, ZF; Tan, W; Wang, SP; Qiang, WA; Wang, YT; Qiang, WA (reprint author), Northwestern Univ, Feinberg Sch Med, Dept Obstet & Gynecol, Div Reprod Sci Med, Chicago, IL 60611 USA.
刊名	SCIENTIFIC REPORTS
	2015-11-10
卷号	5
ISSN号	2045-2322
DOI	10.1038/srep16415
文献子类	Article
英文摘要	Chemo-resistance is the main factor for poor prognosis in human ovarian epithelial cancer. Active constituents derived from Chinese medicine with anti-cancer potential might circumvent this obstacle. In our present study, evodiamine (EVO) derived from Evodia rutaecarpa (Juss.) Benth suppressed the proliferation of human epithelial ovarian cancer, A2780 and the related paclitaxel-resistant cell lines and did not cause cytotoxicity, as confirmed by the significant decline of clone formation and the representative alterations of CFDA-SE fluorescence. Meanwhile, EVO induced cell cycle arrest in a dose-and time-dependent manner. This disturbance might be mediated by the cooperation of Cyclin B1 and Cdc2, including the up-regulation of Cyclin B1, p27, and p21, and activation failure of Cdc2 and pRb. MAPK signaling pathway regulation also assisted in this process. Furthermore, chemo-sensitivity potential was enhanced as indicated in A2780/PTXR cells by the down-regulation of MDR-1 expression, accompanied by MDR-1 function suppression. Taken together, we confirmed initially that EVO exerted an anti-proliferative effect on human epithelial ovarian cancer cells, A2780/WT and A2780/PTXR, induced G2/M phase cell cycle arrest, and improved chemoresistance. Overall, we found that EVO significantly suppressed malignant proliferation in human epithelial ovarian cancer, thus proving to be a potential anti-cancer agent in the future.
学科主题	Science & Technology - Other Topics
出版地	LONDON
项目编号	Macao Science and Technology Development Fund [077/2011/A3, 048/2013/A2] ; Research Fund of University of Macau [CPG2014-00012-ICMS, UL016/09Y4/CMS/WYT01/ICMS, MYRG208 (Y3-L4)-ICMS11-WYT] ; Baskes Foundation ; Robert H. Lurie Comprehensive Cancer Center at the Northwestern University
语种	英语
WOS记录号	WOS:000364384100003
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/179565]
专题	药学院_期刊论文
通讯作者	Qiang, WA (reprint author), Northwestern Univ, Feinberg Sch Med, Dept Obstet & Gynecol, Div Reprod Sci Med, Chicago, IL 60611 USA.
推荐引用方式 GB/T 7714	Zhong, ZF,Tan, W,Wang, SP,et al. Anti-proliferative activity and cell cycle arrest induced by evodiamine on paclitaxel-sensitive and -resistant human ovarian cancer cells[J]. SCIENTIFIC REPORTS,2015,5.
APA	Zhong, ZF,Tan, W,Wang, SP,Qiang, WA,Wang, YT,&Qiang, WA .(2015).Anti-proliferative activity and cell cycle arrest induced by evodiamine on paclitaxel-sensitive and -resistant human ovarian cancer cells.SCIENTIFIC REPORTS,5.
MLA	Zhong, ZF,et al."Anti-proliferative activity and cell cycle arrest induced by evodiamine on paclitaxel-sensitive and -resistant human ovarian cancer cells".SCIENTIFIC REPORTS 5(2015).