A Rapid Hydrophilic Interaction Liquid Chromatographic Method for Simultaneous Determination of Three Ephedrine Alkaloids in Ephedra Herb and Its Preparations

doi:10.1080/10826076.2015.1063509

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	A Rapid Hydrophilic Interaction Liquid Chromatographic Method for Simultaneous Determination of Three Ephedrine Alkaloids in Ephedra Herb and Its Preparations
	Lu, NW; Li, N; Dong, YM; Dong, YM (reprint author), Lanzhou Univ, Sch Pharm, 199 Donggang XiLu, Lanzhou 730000, Gansu, Peoples R China.
刊名	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
	2015-09-14
卷号	38 期号:15 页码:1507-1514
关键词	methylephedrine mechanism hydrophilic interaction liquid chromatography quantitative analysis pseudoephedrine ephedrine
ISSN号	1082-6076
DOI	10.1080/10826076.2015.1063509
文献子类	Article
英文摘要	A rapid hydrophilic interaction liquid chromatographic method has been developed and validated for simultaneous quantitative analysis of methylephedrine, ephedrine, and pseudoephedrine in Ephedra Herb and its preparations. The chromatographic method was performed using a mobile phase consisted of acetonitrile-ammonium acetate (pH 5.0; 0.195M) (95:5, v/v), running at 1.5mL min(-1) on a Pinnacle II Cyano column (5 mu m, 150x4.6mm) at 30 degrees C. The detector wavelength was set at 208nm. The chromatographic method was validated for specificity, linearity and range, limit of detection and quantification, precision, stability, repeatability, and accuracy. The main parameters were specificity (peak purity match factors were >980), linearity (r>0.9996), intra- and inter-day precisions (RSD %, 0.48 similar to 1.70, and RSD %, 0.81 similar to 1.86, respectively), limit of detections and quantifications (29.49 and 98.31ngmL(-1) for methylephedrine; 47.74 and 159.1ngmL(-1) for ephedrine; 121.8 and 406.0ngmL(-1) for pseudoephedrine). The standard addition recoveries were in the range of 86.84 similar to 102.1% and the RSDs of them were <5.23% in samples. The main mechanism was discussed from properties of analytes and optimized process. The results indicated the developed method was rapid (8min), sensitive, and accurate for simultaneous determination of the analytes in Ephedra Herb and its three preparations.
学科主题	Biochemistry & Molecular Biology ; Chemistry
出版地	PHILADELPHIA
资助项目	中央高校基本科研业务费专项资金
项目编号	Fundamental Research Funds for the Central Universities of China [lzujbky-2011-93]
语种	英语
WOS记录号	WOS:000360437800010
资助机构	LZU
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/179559]
专题	药学院_期刊论文
通讯作者	Dong, YM (reprint author), Lanzhou Univ, Sch Pharm, 199 Donggang XiLu, Lanzhou 730000, Gansu, Peoples R China.
推荐引用方式 GB/T 7714	Lu, NW,Li, N,Dong, YM,et al. A Rapid Hydrophilic Interaction Liquid Chromatographic Method for Simultaneous Determination of Three Ephedrine Alkaloids in Ephedra Herb and Its Preparations[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2015,38(15):1507-1514.
APA	Lu, NW,Li, N,Dong, YM,&Dong, YM .(2015).A Rapid Hydrophilic Interaction Liquid Chromatographic Method for Simultaneous Determination of Three Ephedrine Alkaloids in Ephedra Herb and Its Preparations.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,38(15),1507-1514.
MLA	Lu, NW,et al."A Rapid Hydrophilic Interaction Liquid Chromatographic Method for Simultaneous Determination of Three Ephedrine Alkaloids in Ephedra Herb and Its Preparations".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 38.15(2015):1507-1514.