A novel methodology that combined fast chromatography and antioxidant activities screening in Baimai prescription research

doi:10.4028/www.scientific.net/AMR.699.349

CORC > 兰州大学 > 兰州大学 > 公共卫生学院 > 会议论文

	A novel methodology that combined fast chromatography and antioxidant activities screening in Baimai prescription research
	Zhang, Zi Qian; Chen, Xiao Yu; Duan, Yun Xia; Liang, Fang; Liu, Qing Shan; Zhao, Duo Ming; Zhang, Z. Q. (nlqsh@163.com)
	2013
会议日期	February 20, 2013 - February 21, 2013
会议地点	Singapore
关键词	Chromatography Absorption spectroscopy Chemical engineering Dichloromethane Free radicals Materials science Organic solvents Research Absorption spectrometry Anti-oxidant activities Compound prescription Ethnomedicine Fast chromatography Flash chromatography High-speed separation Research and development
卷号	699
DOI	10.4028/www.scientific.net/AMR.699.349
页码	349-353
英文摘要	To tackle the difficult problem of large ethnomedicine compound prescription research in a fast and efficient way, high-speed separation of Baimai prescriptions was performed by automated flash chromatography on 100-g Si flash columns at a flow-rate of 40 mL/min. Petroleum ether, methylene dichloride, ethyl acetate, methanol and water were used in gradient elution solvent system. 69 fractions were obtained as an ethnodrug compounds library. Separation degree of partial fractions were determined by HPLC-UV absorption spectrometry. Antioxidant activities screening of the fractions in 96 well plates was carryied out. Distribution range of fractions with strong scavenging effect of free radical DPPH was identified. Fraction 5-18 and 20 extracted from ethyl acetate exert the strongest antioxidant activities in vitro, and are identified as effective-composite of the prescription in radical scavenging. The results reported here suggest that the methods used may lead to improvements in the research and development of large ethnomedicine compound prescription. © (2013) Trans Tech Publications, Switzerland.
会议录	Advanced Materials Research
会议录出版地	Zurich-Durnten
学科主题	Materials Science ; Engineering Research ; Chemical Engineering ; Organic Compounds ; Chemical Products Generally ; Chemistry
语种	英语
ISSN号	1022-6680
内容类型	会议论文
源URL	[http://ir.lzu.edu.cn/handle/262010/185228]
专题	公共卫生学院_会议论文
通讯作者	Zhang, Z. Q. (nlqsh@163.com)
推荐引用方式 GB/T 7714	Zhang, Zi Qian,Chen, Xiao Yu,Duan, Yun Xia,et al. A novel methodology that combined fast chromatography and antioxidant activities screening in Baimai prescription research[C]. 见:. Singapore. February 20, 2013 - February 21, 2013.