Fiber-modified adenovirus-mediated suicide gene therapy can efficiently eliminate bladder cancer cells in vitro and in vivo

doi:10.18632/oncotarget.12324

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	Fiber-modified adenovirus-mediated suicide gene therapy can efficiently eliminate bladder cancer cells in vitro and in vivo
	Wang, DG; Zhao, MJ; Liu, YQ; Liu, XW; Niu, HT; Song, YF; Tian, YX
刊名	ONCOTARGET
	2016-11-01
卷号	7 期号:44 页码:71710-71717
关键词	gene therapy bladder cancer suicide gene adenovirus
ISSN号	1949-2553
DOI	10.18632/oncotarget.12324
文献子类	Article
英文摘要	Adenovirus-mediated gene therapy is a promising strategy for bladder cancer treatment. However, the loss of the coxsackie and adenovirus receptor (CAR) in bladder cancer cells decreases the infection efficiency of the therapeutic adenovirus. In this study, we constructed an Arg-Gly-Asp (RGD)-modified adenovirus, RGDAd-UPII-TK, that carries a suicide gene called HSV-TK that is driven by a human UPII promoter. Then, we tested the bladder cancer specificity of the UPII promotor and the expression of the HSV-TK protein. Additionally, we observed a potent cytotoxic effects of RGDAd-UPII-TK and ganciclovir (GCV) on bladder cancer as demonstrated by reduced cell survival and morphology changes in vitro. Furthermore, we confirmed that RGDAd-UPII-TK in combination with a GCV injection could significantly reduce the established T24 tumor growth and increase apoptosis in vivo. Altogether, our results indicated that the recombinant adenovirus RGDAd-UPII-TK could target bladder cancer through valid gene therapy.
学科主题	Oncology ; Cell Biology
出版地	ALBANY
资助项目	国家自然科学基金项目
项目编号	National Natural Science Foundation of China [81160287, 81472541] ; Gansu Youth Innovation Talent Support Plan
语种	英语
WOS记录号	WOS:000387449100053
资助机构	NSFC ; GS-OTHER
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/188740]
专题	基础医学院_期刊论文
通讯作者	Song, YF
推荐引用方式 GB/T 7714	Wang, DG,Zhao, MJ,Liu, YQ,et al. Fiber-modified adenovirus-mediated suicide gene therapy can efficiently eliminate bladder cancer cells in vitro and in vivo[J]. ONCOTARGET,2016,7(44):71710-71717.
APA	Wang, DG.,Zhao, MJ.,Liu, YQ.,Liu, XW.,Niu, HT.,...&Tian, YX.(2016).Fiber-modified adenovirus-mediated suicide gene therapy can efficiently eliminate bladder cancer cells in vitro and in vivo.ONCOTARGET,7(44),71710-71717.
MLA	Wang, DG,et al."Fiber-modified adenovirus-mediated suicide gene therapy can efficiently eliminate bladder cancer cells in vitro and in vivo".ONCOTARGET 7.44(2016):71710-71717.