Synthetic Isoliquiritigenin Inhibits Human Tongue Squamous Carcinoma Cells through Its Antioxidant Mechanism

doi:10.1155/2017/1379430

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	Synthetic Isoliquiritigenin Inhibits Human Tongue Squamous Carcinoma Cells through Its Antioxidant Mechanism
	Hou, CL; Li, WG; Li, ZY; Gao, J; Chen, ZJ; Zhao, XQ; Yang, YY; Zhang, XY; Song, Y; Zhang, XY (reprint author), Lanzhou Univ, Sch Basic Med Sci, Lanzhou 730000, Gansu, Peoples R China.
刊名	OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
	2017
ISSN号	1942-0900
DOI	10.1155/2017/1379430
文献子类	Article
英文摘要	Isoliquiritigenin (ISL), a natural antioxidant, has antitumor activity in different types of cancer cells. However the antitumor effect of ISL on human tongue squamous carcinoma cells (TSCC) is not clear. Here we aimed to investigate the effects of synthetic isoliquiritigenin (S-ISL) on TSCC and elucidate the underlying mechanisms. S-ISL was synthesized and elucidated from its nuclear magnetic resonance spectrum and examined using high performance liquid chromatography. The effects of S-ISL on TSCC cells (Tca8113) were evaluated in relation to cell proliferation, apoptosis and adhesion, migration, and invasion using sulforhodamine B assay, fluorescence microscopy technique, flow cytometry (FCM) analysis, and Boyden chamber assay. The associated regulatory mechanisms were examined using FCM and fluorescence microscopy for intracellular reactive oxygen species (ROS) generation, Gelatin zymography assay for matrix metalloproteinase (MMP) activities, and Western blot for apoptosis regulatory proteins (Bcl2 and Bax). Our data indicated that S-ISL inhibited Tca8113 cell proliferation, adhesion, migration, and invasion while promoting the cell apoptosis. Such effects were accompanied by downregulation of Bcl-2 and upregulation of Bax, reduction of MMP-2 and MMP-9 activities, and decreased ROS production. We conclude that S-ISL is a promising agent targeting TSCC through multiple anticancer effects, regulated by its antioxidant mechanism.
学科主题	Cell Biology
出版地	LONDON
资助项目	国家自然科学基金项目 ; 甘肃省自然科学基金计划 ; 兰州市科技局资助项目
项目编号	National Natural Science Foundation of China [21375052] ; Gansu Province Natural Science Foundation of China [1208RJZA231] ; Lanzhou Science and Technology Development Project [06-1-30]
语种	英语
WOS记录号	WOS:000394117000001
资助机构	NSFC ; GSSTD ; LZSTB
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/188738]
专题	基础医学院_期刊论文
通讯作者	Zhang, XY (reprint author), Lanzhou Univ, Sch Basic Med Sci, Lanzhou 730000, Gansu, Peoples R China.; Zhang, XY (reprint author), Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou 730000, Gansu, Peoples R China.; Song, Y (reprint author), Univ Western Australia, Curtin Univ, Ctr Genet Origins Hlth & Dis, Sch Publ Hlth, M409,35 Stirling Highway, Crawley, WA 6009, Australia.; Song, Y (reprint author), Curtin Univ, M409,35 Stirling Highway, Crawley, WA 6009, Australia.
推荐引用方式 GB/T 7714	Hou, CL,Li, WG,Li, ZY,et al. Synthetic Isoliquiritigenin Inhibits Human Tongue Squamous Carcinoma Cells through Its Antioxidant Mechanism[J]. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY,2017.
APA	Hou, CL.,Li, WG.,Li, ZY.,Gao, J.,Chen, ZJ.,...&Song, Y .(2017).Synthetic Isoliquiritigenin Inhibits Human Tongue Squamous Carcinoma Cells through Its Antioxidant Mechanism.OXIDATIVE MEDICINE AND CELLULAR LONGEVITY.
MLA	Hou, CL,et al."Synthetic Isoliquiritigenin Inhibits Human Tongue Squamous Carcinoma Cells through Its Antioxidant Mechanism".OXIDATIVE MEDICINE AND CELLULAR LONGEVITY (2017).