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	Effect of triptolide on expression of thrombospondin-1 and transforming growth factor-beta 1 in renal tubular cells
	Zeng, R; Duan, L; Sun, LN; Kong, YK; Yang, KH; Yang, KH (reprint author), Lanzhou Univ, Sch Basic Med Sci, Evidence Based Med Ctr, Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China.
刊名	RENAL FAILURE
	2015
卷号	37期号:6页码:1039-1043
关键词	Angiotensin-II renal tubular cells renal tubular interstitium thrombospondin 1 transforming growth factor beta 1 triptolide
ISSN号	0886-022X
DOI	10.3109/0886022X.2015.1040705
文献子类	Article
英文摘要	The objective of our study is to investigate the effect of triptolide on expression of thrombospondin-1 and transforming growth factor beta 1 in renal tubular cells. Human renal tubular epithelial cells were stimulated by different concentrations of triptolide (0.1, 1, and 10 mu g/L) in the presence of angiotensin-II (10(-7) mol/L). Real Time PCR was used to detect the mRNA expression of thrombospondin-1 and transforming growth factor beta 1. Western blot analysis was used to detect the protein expression. ELISA was used to detect the level of total and active transforming growth factor beta 1. The mRNA expression of thrombospondin-1 (3.66 +/- 0.48 vs. 1.33 +/- 0.26, p<0.05) and transforming growth factor beta 1 (3.58 +/- 0.59 vs. 1.26 +/- 0.28, p<0.05) were up-regulated obviously when stimulated by angiotensin-II. And the protein expression of thrombospondin-1 (0.5126 +/- 0.0936 vs. 0.1025 +/- 0.0761, p<0.01) and transforming growth factor beta 1 (0.5948 +/- 0.0736 vs. 0.1318 +/- 0.0614, p<0.01) were also upregulated simultaneously when stimulated by angiotensin-II. The expression of thrombospondin-1 and transforming growth factor beta 1 induced by angiotensin-II were down-regulated markedly with 1 mg/L and 10 mg/L of triptolide in mRNA and protein levels (p<0.05, p<0.01). And triptolide (1 and 10 mg/L) could reduce the expression of total and active transforming growth factor beta 1 (p<0.05, p<0.01). In conclusion, triptolide can inhibit the expression of thrombospondin-1 and transforming growth factor beta 1 in mRNA and protein levels and down-regulate the levels of total and active transforming growth factor beta 1.
学科主题	Urology & Nephrology
出版地	ABINGDON
语种	英语
WOS记录号	WOS:000361338600022
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/178997]
专题	基础医学院_期刊论文
通讯作者	Yang, KH (reprint author), Lanzhou Univ, Sch Basic Med Sci, Evidence Based Med Ctr, Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China.
推荐引用方式 GB/T 7714	Zeng, R,Duan, L,Sun, LN,et al. Effect of triptolide on expression of thrombospondin-1 and transforming growth factor-beta 1 in renal tubular cells[J]. RENAL FAILURE,2015,37(6):1039-1043.
APA	Zeng, R,Duan, L,Sun, LN,Kong, YK,Yang, KH,&Yang, KH .(2015).Effect of triptolide on expression of thrombospondin-1 and transforming growth factor-beta 1 in renal tubular cells.RENAL FAILURE,37(6),1039-1043.
MLA	Zeng, R,et al."Effect of triptolide on expression of thrombospondin-1 and transforming growth factor-beta 1 in renal tubular cells".RENAL FAILURE 37.6(2015):1039-1043.

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