Two types of human malignant melanoma cell lines revealed by expression patterns of mitochondrial and survival-apoptosis genes: implications for malignant melanoma therapy

doi:10.1158/1535-7163.MCT-08-1030

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	Two types of human malignant melanoma cell lines revealed by expression patterns of mitochondrial and survival-apoptosis genes: implications for malignant melanoma therapy
	Su, DM; Zhang, QY; Wang, XX; He, P; Zhu, YLJ; Zhao, JX; Rennert, OM; Su, YA; Su, YA (reprint author), George Washington Univ, Sch Med & Hlth Sci, Dept Biochem & Mol Biol, Ross Hall,Room 555,2300 1 St NW, Washington, DC 20037 USA.
刊名	MOLECULAR CANCER THERAPEUTICS
	2009-05
卷号	8 期号:5 页码:1292-1304
ISSN号	1535-7163
DOI	10.1158/1535-7163.MCT-08-1030
文献子类	Article
英文摘要	Human malignant melanoma has poor prognosis because of resistance to apoptosis and therapy. We describe identification of the expression profile of 1,037 mitochondria-focused genes and 84 survival-apoptosis genes in 21 malignant melanoma cell lines and 3 normal melanocyte controls using recently developed hMitChip3 cDNA micro-arrays. Unsupervised hierarchical clustering analysis of 1,037 informative genes, and 84 survival-apoptosis genes, classified these malignant melanoma cell lines into type A (n = 12) and type B (n = 9). Three hundred fifty-five of 1,037 (34.2%) genes displayed significant (P:5 0.030; false discovery rate <= 3.68%) differences ( +/->= 2.0-fold) in average expression, with 197 genes higher and 158 genes lower in type A than in type B. Of 84 genes with known survival-apoptosis functions, 38 (45.2%) displayed the significant (P < 0.001; false discovery rate < 0.15%) difference. Antiapoptotic (BCL2, BCL2A1, PPARD, and RAF1), antioxidant (MT3, PRDX5, PRDX3, GPX4, GLRX2, and GSR), and proapoptotic (BAD, BNIP1, APAF1, BNIP3L, CASP7, CYCS, CASP1, and VDAC1) genes expressed at higher levels in type A than in type B, whereas the different set of antiapoptotic (PSEN1, PPP2CA, API5, PPP2R1B, PPP2R1A, and FIS1), antioxidant [HSPD1, GSS, SOD1, ATOX1, and CAT), and proapoptotic (ENDOG, BAK1, CASP2, CASP4, PDCD5, HTRA2 SEPT4, TNFSF10, and PRODH) genes expressed at lower levels in type A than in type B. Microarray data were validated by quantitative reverse transcription-PCR. These results showed the presence of two types of malignant melanoma, each with a specific set of dysregulated survival-apoptosis genes, which may prove useful for development of new molecular targets for therapeutic intervention and novel diagnostic biomarkers for treatment and prognosis of malignant melanoma. [Mol Cancer Ther 2009;8(5):1292-1304]
学科主题	Oncology
出版地	PHILADELPHIA
语种	英语
WOS记录号	WOS:000266189900033
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/121408]
专题	基础医学院_期刊论文
通讯作者	Su, YA (reprint author), George Washington Univ, Sch Med & Hlth Sci, Dept Biochem & Mol Biol, Ross Hall,Room 555,2300 1 St NW, Washington, DC 20037 USA.
推荐引用方式 GB/T 7714	Su, DM,Zhang, QY,Wang, XX,et al. Two types of human malignant melanoma cell lines revealed by expression patterns of mitochondrial and survival-apoptosis genes: implications for malignant melanoma therapy[J]. MOLECULAR CANCER THERAPEUTICS,2009,8(5):1292-1304.
APA	Su, DM.,Zhang, QY.,Wang, XX.,He, P.,Zhu, YLJ.,...&Su, YA .(2009).Two types of human malignant melanoma cell lines revealed by expression patterns of mitochondrial and survival-apoptosis genes: implications for malignant melanoma therapy.MOLECULAR CANCER THERAPEUTICS,8(5),1292-1304.
MLA	Su, DM,et al."Two types of human malignant melanoma cell lines revealed by expression patterns of mitochondrial and survival-apoptosis genes: implications for malignant melanoma therapy".MOLECULAR CANCER THERAPEUTICS 8.5(2009):1292-1304.