GP7 induces internucleosomal DNA fragmentation independent of caspase activation and DNA fragmentation factor in NB4 cells

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	GP7 induces internucleosomal DNA fragmentation independent of caspase activation and DNA fragmentation factor in NB4 cells
	Qi, SN; Jing, YX; Dong, GX; Chen, Y; Yoshida, A; Ueda, T; Qi, SN (reprint author), Lanzhou Univ, Sch Basic Med, Dept Histol & Embryol, Lanzhou 730000, Peoples R China.
刊名	ONCOLOGY REPORTS
	2007-07
卷号	18 期号:1 页码:273-277
关键词	caspase DNA fragmentation factor 40/caspase-activated DNase apoptosis DNA fragmentation podophyllotoxin leukemia
ISSN号	1021-335X
文献子类	Article
英文摘要	DNA fragmentation into internucleosomal fragments is the best recognized biochemical event of apoptosis. Two major caspase pathways have been identified in the signal transduction leading to DNA fragmentation: the receptor pathway and the mitochondrial pathway. DNA fragmentation factor (DFF) has been identified as a major apoptotic endonuclease in the internucleosomal DNA fragmentation process. However, the potential roles of caspases and DFF in internucleosomal DNA fragmentation induced by specific stimuli still need to be investigated since caspase-independent pathways and nuclease(s) other than DFF also play important roles during this process. In the present study, we investigated the activity of GP7 (4-[4 ''-(2 '',2 '',6 '',6 ''-tetramethyl-1 ''-piperidinyloxy) amino]-4'-demethyl epipodophyllotoxin), a new spin-labeled derivative of podophyllotoxin semi-synthesized by our university, to induce apoptosis of the human leukemia cell line NB4. GP7 induced the release of cytochrome-c from mitochondria, activations of caspase-3, -8, and -9, cleavage of DFF45/inhibitor of caspase-activated DNase, activation of DFF40/caspase-activated DNase, and apoptotic DNA fragmentation in NB4 cells. The broad-spectrum caspase inhibitor zVAD-fmk abrogated GP7-induced caspase-3, -8, and -9 activations but could not inhibit GP7-induced apoptotic DNA fragmentation in NB4 cells. Our findings suggest that GP7-induced apoptotic DNA fragmentation in NB4 cells is independent of caspase activation and DFF, although they are closely involved in this process.
学科主题	Oncology
出版地	ATHENS
语种	英语
WOS记录号	WOS:000247629900042
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/121370]
专题	基础医学院_期刊论文
通讯作者	Qi, SN (reprint author), Lanzhou Univ, Sch Basic Med, Dept Histol & Embryol, Lanzhou 730000, Peoples R China.
推荐引用方式 GB/T 7714	Qi, SN,Jing, YX,Dong, GX,et al. GP7 induces internucleosomal DNA fragmentation independent of caspase activation and DNA fragmentation factor in NB4 cells[J]. ONCOLOGY REPORTS,2007,18(1):273-277.
APA	Qi, SN.,Jing, YX.,Dong, GX.,Chen, Y.,Yoshida, A.,...&Qi, SN .(2007).GP7 induces internucleosomal DNA fragmentation independent of caspase activation and DNA fragmentation factor in NB4 cells.ONCOLOGY REPORTS,18(1),273-277.
MLA	Qi, SN,et al."GP7 induces internucleosomal DNA fragmentation independent of caspase activation and DNA fragmentation factor in NB4 cells".ONCOLOGY REPORTS 18.1(2007):273-277.