Phytoestrogen genistein decreases contractile response of aortic artery in vitro and arterial blood pressure in vivo

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	Phytoestrogen genistein decreases contractile response of aortic artery in vitro and arterial blood pressure in vivo
	Li, HF; Wang, LD; Qu, SY; Li, HF (reprint author), Lanzhou Med Coll, Dept Physiol, Lanzhou 730000, Peoples R China.
刊名	ACTA PHARMACOLOGICA SINICA
	2004-03
卷号	25 期号:3 页码:313-318
关键词	genistein aorta smooth muscle muscle relaxation calcium channel vascular endothelium blood pressure
ISSN号	1671-4083
文献子类	Article
英文摘要	AIM: To determine the mechanisms of effects of phytoestrogen genistein on the contracted rabbit aortic arteries in vitro, and observe the effect of genistein and 17-beta estradiol on mean arterial pressure (MAP) in ovariectomized (OVX) rats. METHODS: (1) Strips of rabbit aortic smooth muscle were suspended in organ baths containing Kreb's solution, and then isometric tension was measured. (2) Female mature Wistar rats underwent a bilateral ovariectomy (OVX). Sham-operated rats (SHAM) were used as controls. After administration of genistein (0.4 mg . kg(-1 .) d(-1), sc), 17-beta estradiol (1 mg . kg(-1) . d(-1), sc) or their vehicle sesame oil for 21 d, MAP was measured. RESULTS: (1) Similar to 17-beta estradiol. genistein could dose-dependently relax 40 mmol/L KCl-precontracted arterial strips. Incubation with N(omega)-L-nitro-arginine (L-NNA), methylene blue (MB), indomethacin, propranolol or endothelium removal did not affect relaxation induced by genistein. In calcium-free solution containing 0.01mmol/L egtazic acid (EGTA), genistein inhibited not only the first phase contraction induced by noradrenaline (NA), but also the second contraction induced by CaCl(2). In addition, genistem could reduce the contractile responses of NA, KCl and CaCl, and shift their cumulative concentration-response curves rightward. (2) MAP in OVX rats was significantly higher compared with that of SHAM rats. However, after chronically treatment with genistein or 17-beta estradiol for 21 d the baseline MAP in OVX rats was reduced significantly. CONCLUSIONS: (1) The vasodilator effect of genistein in vitro is endothelium independent and not related to the nitric oxide, its mechanisms being probably due to inhibition of Ca(2+) influx through calcium channels in a noncompetitive manner and Ca(2+) release from intracellular store induced by NA. (2) Administration of genistein or 17-beta estradiol can chronically decrease MAP in OVX rats.
学科主题	Chemistry ; Pharmacology & Pharmacy
出版地	SHANGHAI
语种	英语
CSCD记录号	CSCD:1844987
WOS记录号	WOS:000220117800011
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/121340]
专题	基础医学院_期刊论文
通讯作者	Li, HF (reprint author), Lanzhou Med Coll, Dept Physiol, Lanzhou 730000, Peoples R China.
推荐引用方式 GB/T 7714	Li, HF,Wang, LD,Qu, SY,et al. Phytoestrogen genistein decreases contractile response of aortic artery in vitro and arterial blood pressure in vivo[J]. ACTA PHARMACOLOGICA SINICA,2004,25(3):313-318.
APA	Li, HF,Wang, LD,Qu, SY,&Li, HF .(2004).Phytoestrogen genistein decreases contractile response of aortic artery in vitro and arterial blood pressure in vivo.ACTA PHARMACOLOGICA SINICA,25(3),313-318.
MLA	Li, HF,et al."Phytoestrogen genistein decreases contractile response of aortic artery in vitro and arterial blood pressure in vivo".ACTA PHARMACOLOGICA SINICA 25.3(2004):313-318.