Carbon Ion-Irradiated Hepatoma Cells Exhibit Coupling Interplay between Apoptotic Signaling and Morphological and Mechanical Remodeling

doi:10.1038/srep35131

	Carbon Ion-Irradiated Hepatoma Cells Exhibit Coupling Interplay between Apoptotic Signaling and Morphological and Mechanical Remodeling
	Zhang, BP; Li, L; Li, ZQ; Liu, Y; Zhang, H; Wang, JZ; Wang, JZ (reprint author), Lanzhou Univ, Coll Civil Engn & Mech, Key Lab Mech Disaster & Environm Western China, Minist Educ, Lanzhou 730000, Peoples R China.; Liu, Y (reprint author), Chinese Acad Sci, Inst Modern Phys, Dept Heavy Radiat Med, Lanzhou 730000, Peoples R China.; Wang, JZ (reprint author), Lanzhou Univ, Inst Biomech & Med Engn, Lanzhou 730000, Peoples R China.
刊名	SCIENTIFIC REPORTS
	2016-10-12
卷号	6
ISSN号	2045-2322
DOI	10.1038/srep35131
文献子类	Article
英文摘要	A apoptotic model was established based on the results of five hepatocellular carcinoma cell (HCC) lines irradiated with carbon ions to investigate the coupling interplay between apoptotic signaling and morphological and mechanical cellular remodeling. The expression levels of key apoptotic proteins and the changes in morphological characteristics and mechanical properties were systematically examined in the irradiated HCC lines. We observed that caspase-3 was activated and that the Bax/Bcl-2 ratio was significantly increased over time. Cellular morphology and mechanics analyses indicated monotonic decreases in spatial sizes, an increase in surface roughness, a considerable reduction in stiffness, and disassembly of the cytoskeletal architecture. A theoretical model of apoptosis revealed that mechanical changes in cells induce the characteristic cellular budding of apoptotic bodies. Statistical analysis indicated that the projected area, stiffness, and cytoskeletal density of the irradiated cells were positively correlated, whereas stiffness and caspase-3 expression were negatively correlated, suggesting a tight coupling interplay between the cellular structures, mechanical properties, and apoptotic protein levels. These results help to clarify a novel arbitration mechanism of cellular demise induced by carbon ions. This biomechanics strategy for evaluating apoptosis contributes to our understanding of cancer-killing mechanisms in the context of carbon ion radiotherapy.
学科主题	Science & Technology - Other Topics
出版地	LONDON
资助项目	国家自然科学基金项目
项目编号	National Natural Science Foundation of China [11032006, 11121202, 11472119, 11421062, 11305224, 11575262]
语种	英语
WOS记录号	WOS:000384991500001
资助机构	NSFC
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/189432]
专题	土木工程与力学学院_期刊论文
通讯作者	Wang, JZ (reprint author), Lanzhou Univ, Coll Civil Engn & Mech, Key Lab Mech Disaster & Environm Western China, Minist Educ, Lanzhou 730000, Peoples R China.; Liu, Y (reprint author), Chinese Acad Sci, Inst Modern Phys, Dept Heavy Radiat Med, Lanzhou 730000, Peoples R China.; Wang, JZ (reprint author), Lanzhou Univ, Inst Biomech & Med Engn, Lanzhou 730000, Peoples R China.
推荐引用方式 GB/T 7714	Zhang, BP,Li, L,Li, ZQ,et al. Carbon Ion-Irradiated Hepatoma Cells Exhibit Coupling Interplay between Apoptotic Signaling and Morphological and Mechanical Remodeling[J]. SCIENTIFIC REPORTS,2016,6.
APA	Zhang, BP.,Li, L.,Li, ZQ.,Liu, Y.,Zhang, H.,...&Wang, JZ .(2016).Carbon Ion-Irradiated Hepatoma Cells Exhibit Coupling Interplay between Apoptotic Signaling and Morphological and Mechanical Remodeling.SCIENTIFIC REPORTS,6.
MLA	Zhang, BP,et al."Carbon Ion-Irradiated Hepatoma Cells Exhibit Coupling Interplay between Apoptotic Signaling and Morphological and Mechanical Remodeling".SCIENTIFIC REPORTS 6(2016).