Exploration of Human Salivary Microbiomes-Insights into the Novel Characteristics of Microbial Community Structure in Caries and Caries-Free Subjects

doi:10.1371/journal.pone.0147039

	Exploration of Human Salivary Microbiomes-Insights into the Novel Characteristics of Microbial Community Structure in Caries and Caries-Free Subjects
	Zhou, JY; Jiang, N; Wang, SG; Hu, XP; Jiao, KL; He, XY; Li, ZQ; Wang, JZ; Wang, JZ (reprint author), Lanzhou Univ, Coll Civil Engn & Mech, Lanzhou 730000, Gansu, Peoples R China.; Li, ZQ (reprint author), Northwest Univ Nationalities, Sch Stomatol, Key Lab Oral Dis Gansu Prov, Lanzhou 730030, Gansu, Peoples R China.
刊名	PLOS ONE
	2016-01-19
卷号	11 期号:1
ISSN号	1932-6203
DOI	10.1371/journal.pone.0147039
文献子类	Article
英文摘要	Recently, high-throughput sequencing has improved the understanding of the microbiological etiology of caries, but the characteristics of the microbial community structure in the human oral cavity with and without caries are not completely clear. To better understand these characteristics, Illumina MiSeq high-throughput sequencing was utilized to analyze 20 salivary samples (10 caries-free and 10 caries) from subjects from the same town in Dongxiang, Gansu, China. A total of 5,113 OTUs (Operational Taxonomic Units, 97% cutoff) were characterized in all of the salivary samples obtained from the 20 subjects. A comparison of the two groups revealed that (i) the predominant phyla were constant between the two groups; (ii) the relative abundance of the genera Veillonella, Bifidobacterium, Selenomonas, Olsenella, Parascardovia, Scardovia, Chryseobacterium, Terrimonas, Burkholderia and Sporobacter was significantly higher in the group with caries (P < 0.05); and (iii) four genera with low relative abundance (< 0.01% on average), including two characteristic genera in caries (Chryseobacterium and Scardovia), significantly influenced the microbial community structure at the genus and OTU levels. Moreover, via co-occurrence and principal component analyses, the co-prevalence of the pathogenic genera was detected in the caries samples, but in the caries-free samples, the function of clustered genera was more random. This result suggests that a synergistic effect may be influencing the assembly of the caries microbial community, whereas competition may play a more dominant role in governing the microbial community in the caries-free group. Our findings regarding the characteristics of the microbial communities of the groups with and without caries might improve the understanding of the microbiological etiology of caries and might improve the prevention and cure of caries in the future.
学科主题	Science & Technology - Other Topics
出版地	SAN FRANCISCO
资助项目	国家自然科学基金项目 ; 甘肃省国际科技合作计划
项目编号	National Natural Science Fund [31160124/C0309, 31360124/C0309] ; Special International Technology Cooperation of Gansu Province [144WCGA167]
语种	英语
WOS记录号	WOS:000368528400099
资助机构	NSFC ; GSSTD
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/182491]
专题	土木工程与力学学院_期刊论文
通讯作者	Wang, JZ (reprint author), Lanzhou Univ, Coll Civil Engn & Mech, Lanzhou 730000, Gansu, Peoples R China.; Li, ZQ (reprint author), Northwest Univ Nationalities, Sch Stomatol, Key Lab Oral Dis Gansu Prov, Lanzhou 730030, Gansu, Peoples R China.
推荐引用方式 GB/T 7714	Zhou, JY,Jiang, N,Wang, SG,et al. Exploration of Human Salivary Microbiomes-Insights into the Novel Characteristics of Microbial Community Structure in Caries and Caries-Free Subjects[J]. PLOS ONE,2016,11(1).
APA	Zhou, JY.,Jiang, N.,Wang, SG.,Hu, XP.,Jiao, KL.,...&Li, ZQ .(2016).Exploration of Human Salivary Microbiomes-Insights into the Novel Characteristics of Microbial Community Structure in Caries and Caries-Free Subjects.PLOS ONE,11(1).
MLA	Zhou, JY,et al."Exploration of Human Salivary Microbiomes-Insights into the Novel Characteristics of Microbial Community Structure in Caries and Caries-Free Subjects".PLOS ONE 11.1(2016).