Porcine LEM domain-containing 3: Molecular cloning, functional characterization, and polymorphism associated with ear size

doi:10.1016/S2095-3119(15)61173-X

CORC > 中国农业科学院 > 北京畜牧兽医研究所

	Porcine LEM domain-containing 3: Molecular cloning, functional characterization, and polymorphism associated with ear size
	Liang Jing 1; Li Na 1; Zhang Longchao 1; Wang Ligang 1; Liu Xin 1; Zhao Kebin 1; Yan Hua 1; Pu Lei 1; Zhang Yuebo 1; Shi Huibi 1
刊名	Journal of Integrative Agriculture
	2016
卷号	15 期号:6 页码:1321-1329
关键词	association analysis ear size LEMD3 molecular cloning pig
ISSN号	2095-3119
DOI	10.1016/S2095-3119(15)61173-X
英文摘要	Ear size exhibits remarkable diversity in pig breeds. LEM domain-containing 3 (LEMD3) on chromosome 5 is considered as an important candidate for porcine ear size. This is the first study on cloning and characterization of LEMD3 cDNA. The complete cDNA contains 4 843 bp, including a 2 736-bp open reading frame (ORF), a 37-bp 5'-untranslated region (UTR) and a 2 070-bp 3'-UTR. The complete LEMD3 gene is 126 241-bp and contains 13 exons and 12 introns. The ORF encodes a deduced LEMD3 protein of 911 amino acids, which shares 82-94% nucleic acid and 51-96% amino acid identity with other species. A phylogenetic tree constructed based on the amino acid sequences revealed that the porcine LEMD3 protein was closely related with cattle LEMD3. Resequencing of the ORF and promoter of LEMD3 from Minzhu pig and Large White revealed three single nucleotide polymorphisms (SNPs): L964C>A in the complete coding region, L4625A>G in the 3' UTR, and L-394T>C in the promoter region. Genome-wide association study (GWAS) revealed that all of SNPs were shown significant association with ear size in Large White譓inzhu pig intercross population. With conditional GWAS,-log_(10)(P-value) decreased by more than 80% when each of three SNPs was included as a fixed effect. These results suggested direct involvement of LEMD3 or close linkage to the causative mutation for ear size. The findings of this study might form the basis for understanding the genetic mechanism of ear size variation in pigs and provide potential molecular markers for screening ear size diversity in pig breeds.
学科主题	畜牧、动物医学、狩猎、蚕、蜂
语种	中文
内容类型	期刊论文
源URL	[http://ir.nais.net.cn/handle/2HMLN22E/119663]
专题	北京畜牧兽医研究所
作者单位	1.Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation, Ministry of Agriculture, Beijing, 100193; 2.College of Animal Science and Technology, China Agricultural University, Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture, Beijing, 100193
推荐引用方式 GB/T 7714	Liang Jing,Li Na,Zhang Longchao,et al. Porcine LEM domain-containing 3: Molecular cloning, functional characterization, and polymorphism associated with ear size[J]. Journal of Integrative Agriculture,2016,15(6):1321-1329.
APA	Liang Jing.,Li Na.,Zhang Longchao.,Wang Ligang.,Liu Xin.,...&Wang Lixian.(2016).Porcine LEM domain-containing 3: Molecular cloning, functional characterization, and polymorphism associated with ear size.Journal of Integrative Agriculture,15(6),1321-1329.
MLA	Liang Jing,et al."Porcine LEM domain-containing 3: Molecular cloning, functional characterization, and polymorphism associated with ear size".Journal of Integrative Agriculture 15.6(2016):1321-1329.