Direct electron transfer to cytochrome c oxidase in self-assembled monolayers on gold electrodes

	Direct electron transfer to cytochrome c oxidase in self-assembled monolayers on gold electrodes
	Li JH ; Cheng GJ ; Dong SJ
刊名	journal of electroanalytical chemistry
	1996
卷号	416 期号:1-2 页码:97-104
关键词	ORGANIZED MOLECULAR ASSEMBLIES DIRECT ELECTROCHEMISTRY THIOL MONOLAYERS REDOX PROTEINS SURFACES ADSORPTION SPECTROSCOPY SYSTEMS BINDING CHEMISORPTION
ISSN号	0022-0728
中文摘要	the monolayer of cytochrome c oxidase maintaining physiological activity and attached covalently to the self-assembled monolayers of 3-mercaptopropionic acid (mpa) on a gold electrode was obtained. the results of cyclic voltammetry show that direct electron transfer between cytochrome c oxidase and the electrode surface is a fast and diffusionless process. mpa has a dual role as both electrode modifier and the bridging molecule which: keeps cytochrome c oxidase at an appropriate orientation without denaturation and enables direct electron transfer between the protein and the modified electrode. immobilized cytochrome c oxidase exhibits biphasic phenomena between the concentration of the electrolyte and the normal potentials; meanwhile its electrochemical behavior is also influenced by the buffer components. the quasi-reversible electron transfer process of cytochrome c oxidase with formal potential 385 mv vs. she in 5mm phosphate buffer solution (ph 6.4) corresponds to the redox reaction of cyt a(3) in cytochrome c oxidase, and the heterogeneous electron transfer rate constant obtained is 1.56 s(-1). by cyclic voltammetry measurements, it was observed that oxidation and reduction of cytochrome c in solution were catalyzed by the immobilized cytochrome c oxidase. this cytochrome c oxidase/mpa/au system provides a good mimetic model to study the physiological functions of membrane-associated enzymes and hopefully to build a third-generation biosensor without using a mediator.
收录类别	SCI收录期刊论文
语种	英语
公开日期	2010-12-22 ; 2011-06-09
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/25161]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Li JH,Cheng GJ,Dong SJ. Direct electron transfer to cytochrome c oxidase in self-assembled monolayers on gold electrodes[J]. journal of electroanalytical chemistry,1996,416(1-2):97-104.
APA	Li JH,Cheng GJ,&Dong SJ.(1996).Direct electron transfer to cytochrome c oxidase in self-assembled monolayers on gold electrodes.journal of electroanalytical chemistry,416(1-2),97-104.
MLA	Li JH,et al."Direct electron transfer to cytochrome c oxidase in self-assembled monolayers on gold electrodes".journal of electroanalytical chemistry 416.1-2(1996):97-104.