马铃薯茎线虫的培养

	马铃薯茎线虫的培养
	徐进军 1; 李世东 1; 杨之为 2
刊名	西北农业学报
	2004
卷号	13 期号:4 页码:77-80
关键词	马铃薯茎线虫茄腐镰刀菌培养
ISSN号	1004-1389
其他题名	Culture of Potato Rot Nemotode
英文摘要	采用茄腐镰刀菌(Fusarium solani)培养马铃薯茎线虫,在20℃黑暗条件下培养43d,直径9 cm的培养皿每皿活线虫总数平均为23,777条/皿;在25℃黑暗条件下培养周期43d,每皿一次可培养出46,000至72,000条线虫,平均60,777条.与Young和Seung的培养结果(10,000条/皿)[1]相比每皿多50,000条左右,而且培养的线虫聚集在培养皿盖上,方法简单,培养量大,含杂质少.; This paper reported an improved method to harvest a large number of Ditylenchus desturctor, pathogen of sweet potato rot disease, in laboratory condition. The nematode was cultured for 43d on colony of Fusarium solani at 20"Cunder darkness, leading to 23,777 nematodes reproduced per cover of Petri dish (dia. 9cm), at 25'C leading to 46,000-72,000 reproduced. Comparing wjth Young Ho Kim&. Seung Hwan Ohh's method, this method is not only more efficient but also more simple, and the nematodes gained is more clean because they moved into the inside of the Petri dishes cover and congregated together there.
学科主题	植物保护
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/17575]
专题	农业环境与可持续发展研究所
作者单位	1.中国农业科学院生物防治研究所, 北京, 100081 2.西北农林科技大学植物保护学院, 陕西, 杨凌, 712100
推荐引用方式 GB/T 7714	徐进军,李世东,杨之为. 马铃薯茎线虫的培养[J]. 西北农业学报,2004,13(4):77-80.
APA	徐进军,李世东,&杨之为.(2004).马铃薯茎线虫的培养.西北农业学报,13(4),77-80.
MLA	徐进军,et al."马铃薯茎线虫的培养".西北农业学报 13.4(2004):77-80.