Gateway技术构建交链孢菌JH505 cDNA文库

	Gateway技术构建交链孢菌JH505 cDNA文库
	龙松华 1; 张宁 1; 邱德文 1; 黎定军 2; 黄炜 1
刊名	微生物学报
	2005
卷号	45 期号:6 页码:963-965
关键词	Gateway技术交链孢菌激活蛋白 cDNA入门文库 cDNA表达文库
ISSN号	0001-6209
其他题名	Construction of a cDNA library of Alternaria sp. strain JH505 using Gateway technology
英文摘要	Gateway技术构建Cdna文库,利用λ噬菌体的位点特异性重组,避免使用限制性内酶切割Cdna,能够解决常规方法构建Cdna文库的技术缺陷.首次应用Gateway技术构建交链孢菌Cdna文库,经检测Cdna入门文库的滴度达到1×10^7cfu/Ml,文库总容量为9×10^7cfu,平均插入片段为1510bp.通过LR重组把入门文库转换为表达文库,表达文库的滴度为1.58×10^6cfu/Ml,文库总容量为6.32×10^6cfu,平均插入片段大小为1680bp.表达文库的构建为进一步克隆植物激活蛋白基因打下了基础.; A cDNA library was constructed based on the Gateway system that is a method to construct a high-quality cDNA library without the use of restriction enzyme for cloning. This is the first report that the Gateway system is used to construct a cDNA library for Alternaria sp.. The entry cDNA library was constructed in this report has a high titer of 1× 10^7cfu/mL and contain a total clones of 9× 10^7cfu, with an average inserts size of about 1510bp. In order to screen for a gene encoding the plant activator protein from library using an antiserum, the entry cDNA library was transferred into Gateway destination vector to create an expression library. The expression library show a high titer of 1.58 × 10^6cfu/mL and contains a total clones of 6.32 × 10^6cfu, with an average inserts size of 1680bp.
学科主题	分子生物学
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/17521]
专题	农业环境与可持续发展研究所
作者单位	1.中国农业科学院农业环境与可持续发展研究所, 北京, 100081 2.湖南农业大学生物安全科技学院, 湖南, 长沙, 410128
推荐引用方式 GB/T 7714	龙松华,张宁,邱德文,等. Gateway技术构建交链孢菌JH505 cDNA文库[J]. 微生物学报,2005,45(6):963-965.
APA	龙松华,张宁,邱德文,黎定军,&黄炜.(2005).Gateway技术构建交链孢菌JH505 cDNA文库.微生物学报,45(6),963-965.
MLA	龙松华,et al."Gateway技术构建交链孢菌JH505 cDNA文库".微生物学报 45.6(2005):963-965.