Chloroplast and mitochondrial SSR help to distinguish allo-cytoplasmic male sterile types in cabbage (Brassica oleracea L. var. capitata)

doi:10.1007/s11032-011-9656-9

	Chloroplast and mitochondrial SSR help to distinguish allo-cytoplasmic male sterile types in cabbage (Brassica oleracea L. var. capitata)
	Wang, Qingbiao; Zhang, Yangyong; Fang, Zhiyuan; Liu, Yumei; Yang, Limei; Zhuang, Mu
刊名	MOLECULAR BREEDING
	2012
卷号	30 期号:2 页码:709-716
关键词	Cabbage Cytoplasmic male sterility (CMS) Mitochondrial SSR (mtSSR) Chloroplast SSR (cpSSR)
ISSN号	1380-3743
DOI	10.1007/s11032-011-9656-9
通讯作者	Wang, Qingbiao
英文摘要	The profiles of single sequence repeat (SSR) in six distinct allo-cytoplasmic male sterile (CMS) types of cabbage (Brassica oleracea L. var. capitata) were generated using 32 SSR primer pairs derived from the Arabidopsis thaliana chloroplast (cp) genome and another 21 SSR primers from the B. napus mitochondrial (mt) genome sequences. In total, 11 cpSSR and 4 mtSSR primers revealed polymorphism among the six cabbage CMS types, namely NigCMS, OguCMSR(1), OguCMSR(2), OguCMSR(3), OguCMSHY and PolCMS. Through cluster analysis, six cabbage CMS types could be unambiguously differentiated with just three sets of primers (ACP43, ACP47, mtSSR2). Analysis of the selected amplicon sequences showed high identity to that of the corresponding sequences in A. thaliana, B. rapa and B. napus. The aligned cluster analysis revealed that the polymorphism mainly included SSR number variation, single nucleotide polymorphism (SNP), and sequence insertion or deletion (InDel). Our results demonstrated that specific mitochondrial or chloroplast SSR analysis could be a feasible alternative means for cabbage CMS type identification.
学科主题	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture ; AGRONOMY ; GENETICS & HEREDITY ; HORTICULTURE ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000307270900008
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101094]
专题	蔬菜花卉研究所_十字花科研究室
作者单位	Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Wang, Qingbiao,Zhang, Yangyong,Fang, Zhiyuan,et al. Chloroplast and mitochondrial SSR help to distinguish allo-cytoplasmic male sterile types in cabbage (Brassica oleracea L. var. capitata)[J]. MOLECULAR BREEDING,2012,30(2):709-716.
APA	Wang, Qingbiao,Zhang, Yangyong,Fang, Zhiyuan,Liu, Yumei,Yang, Limei,&Zhuang, Mu.(2012).Chloroplast and mitochondrial SSR help to distinguish allo-cytoplasmic male sterile types in cabbage (Brassica oleracea L. var. capitata).MOLECULAR BREEDING,30(2),709-716.
MLA	Wang, Qingbiao,et al."Chloroplast and mitochondrial SSR help to distinguish allo-cytoplasmic male sterile types in cabbage (Brassica oleracea L. var. capitata)".MOLECULAR BREEDING 30.2(2012):709-716.