Linkage map construction using InDel and SSR markers and QTL analysis of heading traits in Brassica oleracea var. capitata L.

doi:10.1007/s11032-014-0019-1

	Linkage map construction using InDel and SSR markers and QTL analysis of heading traits in Brassica oleracea var. capitata L.
	Lv, Honghao; Wang, Qingbiao; Zhang, Yangyong; Yang, Limei; Fang, Zhiyuan; Wang, Xiaowu; Liu, Yumei; Zhuang, Mu; Lin, Yan; Yu, Hailong
刊名	MOLECULAR BREEDING
	2014
卷号	34 期号:1 页码:87-98
关键词	Brassica oleracea Linkage map Quantitative trait loci Heading traits
ISSN号	1380-3743
DOI	10.1007/s11032-014-0019-1
通讯作者	Lv, Honghao
英文摘要	Cabbage heading traits are important quantitative traits that greatly affect both quality and yield of cabbage. However, the genetic control of these traits remains unclear. To detect quantitative trait loci (QTLs) associated with heading traits, a double haploid (DH) population with 196 lines was created from a cabbage hybrid 01-20 x 96-100. A genetic map with insertion-deletion and simple sequence repeat markers was constructed based on the DH population, with a total length of 934.06 cM and average interval length of 2.3 cM between adjacent markers. Field experiments in three seasons were carried out to evaluate the heading traits, including head mature period (Hm), head weight (Hw), core length (Cl), head vertical diameter (Hvd), and the ratio of Cl to Hvd (Cl/Hvd). Using the map and the trait data, 13 reliable QTLs in total were identified and 5 were found in more than one season based on the adjusted means of three seasons. Major QTLs were identified for Hm (R (2) = 40.4, LOD = 14.84), Hw (R (2) = 28.6, LOD = 9.83), Cl (R (2) = 38.8, LOD = 15.73), Hvd (R (2) = 19.2, LOD = 9.26), and Cl/Hvd (R (2) = 38.8, LOD = 12.75). The most significant QTLs were Hm3.1, Cl3.1, and Cl/Hvd3.1, which were detected in three seasons with the maximum contribution rate of almost 40 %. Six active regions that harbored more than one QTL were identified on five chromosomes, and one of them contained major QTLs associated with five traits. The QTLs obtained in this study should be useful for marker-assisted selection in cabbage breeding and for understanding the genetic control of these traits.
学科主题	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture ; AGRONOMY ; GENETICS & HEREDITY ; HORTICULTURE ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000336432100008
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100974]
专题	蔬菜花卉研究所_十字花科研究室
作者单位	Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Lv, Honghao,Wang, Qingbiao,Zhang, Yangyong,et al. Linkage map construction using InDel and SSR markers and QTL analysis of heading traits in Brassica oleracea var. capitata L.[J]. MOLECULAR BREEDING,2014,34(1):87-98.
APA	Lv, Honghao.,Wang, Qingbiao.,Zhang, Yangyong.,Yang, Limei.,Fang, Zhiyuan.,...&Liu, Bo.(2014).Linkage map construction using InDel and SSR markers and QTL analysis of heading traits in Brassica oleracea var. capitata L..MOLECULAR BREEDING,34(1),87-98.
MLA	Lv, Honghao,et al."Linkage map construction using InDel and SSR markers and QTL analysis of heading traits in Brassica oleracea var. capitata L.".MOLECULAR BREEDING 34.1(2014):87-98.