Quantitative Proteomic Profiling of Early and Late Responses to Salicylic Acid in Cucumber Leaves

doi:10.1371/journal.pone.0161395

	Quantitative Proteomic Profiling of Early and Late Responses to Salicylic Acid in Cucumber Leaves
	Dong, Chun-Juan; Cao, Ning; Li, Liang; Shang, Qing-Mao
刊名	PLOS ONE
	2016
卷号	11 期号:8 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0161395
通讯作者	Dong, Chun-Juan
英文摘要	Salicylic acid (SA) is an important phytohormone that plays vital regulatory roles in plant growth, development, and stress responses. However, studies on the molecular mechanism of SA, especially during the early SA responses, are lagging behind. In this study, we initiated a comprehensive isobaric tag for relative and absolute quantitation (iTRAQ)-based proteomic analysis to explore the early and late SA-responsive proteins in leaves of cucumber (Cucumis sativus L.) seedlings. Upon SA application through the roots, endogenous SA accumulated in cucumber leaves. By assaying the changes in marker gene expression and photosynthetic rate, we collected samples at 12 h and 72 h post treatment (hpt) to profile the early and late SA responsiveness, respectively. The iTRAQ assay followed by tandem mass spectrometry revealed 135 differentially expressed proteins (DEPs) at 12 hpt and 301 DEPs at 72 hpt. The functional categories for these SA-responsive proteins included in a variety of biochemical processes, including photosynthesis, redox homeostasis, carbohydrate and energy metabolism, lipid metabolism, transport, protein folding and modification, proteolysis, cell wall organization, and the secondary phenylpropanoid pathway. Conclusively, based on the abundant changes of these DEPs, together with their putative functions, we proposed a possible SA-responsive protein network. It appears that SA could elicit reactive oxygen species (ROS) production via enhancing the photosynthetic electron transferring, and then confer some growth-promoting and stress-priming effects on cells during the late phase, including enhanced photosynthesis and ROS scavenging, altered carbon metabolic flux for the biosynthesis of amino acids and nucleotides, and cell wall reorganization. Overall, the present iTRAQ assay provides higher proteome coverage and deepened our understanding of the molecular basis of SA-responses.
学科主题	Multidisciplinary Sciences
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000381768800036
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/105911]
专题	蔬菜花卉研究所_栽培与采后技术研究室
作者单位	Chinese Acad Agr Sci, Inst Vegetables & Flowers, Key Lab Hort Crop Biol & Germplasm Innovat, Minist Agr, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Dong, Chun-Juan,Cao, Ning,Li, Liang,et al. Quantitative Proteomic Profiling of Early and Late Responses to Salicylic Acid in Cucumber Leaves[J]. PLOS ONE,2016,11(8):-.
APA	Dong, Chun-Juan,Cao, Ning,Li, Liang,&Shang, Qing-Mao.(2016).Quantitative Proteomic Profiling of Early and Late Responses to Salicylic Acid in Cucumber Leaves.PLOS ONE,11(8),-.
MLA	Dong, Chun-Juan,et al."Quantitative Proteomic Profiling of Early and Late Responses to Salicylic Acid in Cucumber Leaves".PLOS ONE 11.8(2016):-.