Leaf Volatile Compounds and Associated Gene Expression during Short-Term Nitrogen Deficient Treatments in Cucumis Seedlings

doi:10.3390/ijms17111713

	Leaf Volatile Compounds and Associated Gene Expression during Short-Term Nitrogen Deficient Treatments in Cucumis Seedlings
	Deng, Jie 1; Yu, Hong-Jun 1; Li, Yun-Yun 1; Zhang, Xiao-Meng 1; Liu, Peng 1; Li, Qiang 1; Jiang, Wei-Jie 1,2
刊名	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
	2016
卷号	17 期号:11 页码:-
关键词	nitrogen deficiency cucumber leaf volatile aldehyde LOX-HPL pathways
ISSN号	1422-0067
DOI	10.3390/ijms17111713
通讯作者	Deng, Jie
英文摘要	Nitrogen (N) is an important macronutrient for plant growth and development, but the regulatory mechanism of volatile compounds in response to N deficiency is not well understood, especially in cucumber, which consumes excessive N during growth. In this study, the major volatile compounds from cucumber leaves subjected to N deficiency were analyzed by GC-MS. A total of 24 volatile components were identified including 15 aldehydes, two ketones, two alkenes, and five other volatile compounds in 9930 leaves. Principal component analysis using volatile compounds from cucumber leaves provided good separation between N-sufficient and N-deficient treatments. The main volatiles in cucumber leaves were found to be C6 and C9 aldehydes, especially (E)-2-hexanal and (E, Z)-2,6-nonadienal. (E)-2-hexanal belonged to the C6 aldehyde and was the most abundant compound, whereas (E, Z)-2,6-nonadienal was the chief component of C9 aldehydes. During N-deficient treatment, short-chain volatile content was significantly improved at 5 day, other volatiles displayed significant reduction or no significantly changes in all sampling points. Improvement of short-chain volatiles was confirmed in the six other inbred lines at 5 day after N-deficient treatments. The expression analysis of 12 cucumber LOX genes and two HPL genes revealed that CsLOX19, CsLOX20, and CsLOX22 had common up-regulated expression patterns in response to N-deficient stress in most inbred lines; meanwhile, most sample points of CsHPL1 also had significant up-regulated expression patterns. This research focused on the relationship between volatiles in cucumber and different nitrogen environments to provide valuable insight into the effect of cultivation and management of the quality of cucumber and contributes to further research on volatile metabolism in cucumber.
学科主题	Chemistry, Multidisciplinary
语种	英语
出版者	MDPI AG
WOS记录号	WOS:000388809600004
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/105894]
专题	蔬菜花卉研究所_栽培与采后技术研究室
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Key Lab Hort Crops Genet Improvement, Minist Agr, Beijing 100081, Peoples R China 2.Xinjiang Agr Univ, Coll Forestry & Hort, Urumqi 830052, Peoples R China
推荐引用方式 GB/T 7714	Deng, Jie,Yu, Hong-Jun,Li, Yun-Yun,et al. Leaf Volatile Compounds and Associated Gene Expression during Short-Term Nitrogen Deficient Treatments in Cucumis Seedlings[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2016,17(11):-.
APA	Deng, Jie.,Yu, Hong-Jun.,Li, Yun-Yun.,Zhang, Xiao-Meng.,Liu, Peng.,...&Jiang, Wei-Jie.(2016).Leaf Volatile Compounds and Associated Gene Expression during Short-Term Nitrogen Deficient Treatments in Cucumis Seedlings.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,17(11),-.
MLA	Deng, Jie,et al."Leaf Volatile Compounds and Associated Gene Expression during Short-Term Nitrogen Deficient Treatments in Cucumis Seedlings".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 17.11(2016):-.