Fine genetic mapping localizes cucumber scab resistance gene Ccu into an R gene cluster

doi:10.1007/s00122-010-1487-2

	Fine genetic mapping localizes cucumber scab resistance gene Ccu into an R gene cluster
	Kang, Houxiang 1,3; Weng, Yiqun 2; Yang, Yuhong 1,3; Zhang, Zhonghua 1,3; Zhang, Shengping 1,3; Mao, Zhenchuan 1,3; Cheng, Guohua 1,3; Gu, Xingfang 1,3; Huang, Sanwen 1,3; Xie, Bingyan 1,3
刊名	THEORETICAL AND APPLIED GENETICS
	2011
卷号	122 期号:4 页码:795-803
ISSN号	0040-5752
DOI	10.1007/s00122-010-1487-2
通讯作者	Kang, Houxiang
英文摘要	Scab, caused by Cladosporium cucumerinum, is an important disease of cucumber, Cucumis sativus. In this study, we conducted fine genetic mapping of the single dominant scab resistance gene, Ccu, with 148 F(9) recombinant inbred lines (RILs) and 1,944 F(2) plants derived from the resistant cucumber inbred line 9110Gt and the susceptible line 9930, whose draft genome sequence is now available. A framework linkage map was first constructed with simple sequence repeat markers placing Ccu into the terminal 670 kb region of cucumber Chromosome 2. The 9110Gt genome was sequenced at 5x genome coverage with the Solexa next-generation sequencing technology. Sequence analysis of the assembled 9110Gt contigs and the Ccu region of the 9930 genome identified three insertion/deletion (Indel) markers, Indel01, Indel02, and Indel03 that were closely linked with the Ccu locus. On the high-resolution map developed with the F(2) population, the two closest flanking markers, Indel01 and Indel02, were 0.14 and 0.15 cM away from the target gene Ccu, respectively, and the physical distance between the two markers was approximately 140 kb. Detailed annotation of the 180 kb region harboring the Ccu locus identified a cluster of six resistance gene analogs (RGAs) that belong to the nucleotide binding site (NBS) type R genes. Four RGAs were in the region delimited by markers Indel01 and Indel02, and thus were possible candidates of Ccu. Comparative DNA analysis of this cucumber Ccu gene region with a melon (C. melo) bacterial artificial chromosome (BAC) clone revealed a high degree of micro-synteny and conservation of the RGA tandem repeats in this region.
学科主题	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture ; AGRONOMY ; GENETICS & HEREDITY ; HORTICULTURE ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000287244600012
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101157]
专题	蔬菜花卉研究所_植物保护研究室
作者单位	1.Minist Agr, Key Lab Hort Crops Genet Improvement, Beijing 100081, Peoples R China 2.Univ Wisconsin, Dept Hort, USDA ARS Vegetable, Crops Res Unit, Madison, WI 53706 USA 3.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Kang, Houxiang,Weng, Yiqun,Yang, Yuhong,et al. Fine genetic mapping localizes cucumber scab resistance gene Ccu into an R gene cluster[J]. THEORETICAL AND APPLIED GENETICS,2011,122(4):795-803.
APA	Kang, Houxiang.,Weng, Yiqun.,Yang, Yuhong.,Zhang, Zhonghua.,Zhang, Shengping.,...&Xie, Bingyan.(2011).Fine genetic mapping localizes cucumber scab resistance gene Ccu into an R gene cluster.THEORETICAL AND APPLIED GENETICS,122(4),795-803.
MLA	Kang, Houxiang,et al."Fine genetic mapping localizes cucumber scab resistance gene Ccu into an R gene cluster".THEORETICAL AND APPLIED GENETICS 122.4(2011):795-803.