Analysis of the complete mitochondrial genome of Pochonia chlamydosporia suggests a close relationship to the invertebrate-pathogenic fungi in Hypocreales

doi:10.1186/s12866-015-0341-8

	Analysis of the complete mitochondrial genome of Pochonia chlamydosporia suggests a close relationship to the invertebrate-pathogenic fungi in Hypocreales
	Lin, Runmao 1; Liu, Chichuan 1; Shen, Baoming 1,2; Bai, Miao 3; Ling, Jian 1; Chen, Guohua 1; Mao, Zhenchuan 1; Cheng, Xinyue 4; Xie, Bingyan 1
刊名	BMC MICROBIOLOGY
	2015
卷号	15 页码:-
关键词	Nematode egg-parasite fungus Pochonia chlamydosporia Biological control agent Mitochondrial genome rps3 Rearrangement Evolution Phylogeny
ISSN号	1471-2180
DOI	10.1186/s12866-015-0341-8
通讯作者	Lin, Runmao
英文摘要	Background: The fungus Pochonia chlamydosporia parasitizes nematode eggs and has become one of the most promising biological control agents (BCAs) for plant-parasitic nematodes, which are major agricultural pests that cause tremendous economic losses worldwide. The complete mitochondrial (mt) genome is expected to open new avenues for understanding the phylogenetic relationships and evolution of the invertebrate-pathogenic fungi in Hypocreales.Results: The complete mitogenome sequence of Pochonia chlamydosporia is 25,615 bp in size, containing the 14 typical protein-coding genes, two ribosomal RNA genes, an intronic ORF coding for a putative ribosomal protein (rps3) and a set of 23 transfer RNA genes (trn) which recognize codons for all amino acids. Sequence similarity studies and syntenic gene analyses show that 87.02% and 58.72% of Pochonia chlamydosporia mitogenome sequences match 90.50% of Metarhizium anisopliae sequences and 61.33% of Lecanicillium muscarium sequences with 92.38% and 86.04% identities, respectively. A phylogenetic tree inferred from 14 mt proteins in Pezizomycotina fungi supports that Pochonia chlamydosporia is most closely related to the entomopathogenic fungus Metarhizium anisopliae. The invertebrate-pathogenic fungi in Hypocreales cluster together and clearly separate from a cluster comprising plant-pathogenic fungi (Fusarium spp.) and Hypocrea jecorina. A comparison of mitogenome sizes shows that the length of the intergenic regions or the intronic regions is the major size contributor in most of mitogenomes in Sordariomycetes. Evolutionary analysis shows that rps3 is under positive selection, leading to the display of unique evolutionary characteristics in Hypocreales. Moreover, the variability of trn distribution has a clear impact on gene order in mitogenomes. Gene rearrangement analysis shows that operation of transposition drives the rearrangement events in Pezizomycotina, and most events involve in trn position changes, but no rearrangement was found in Clavicipitaceae.Conclusions: We present the complete annotated mitogenome sequence of Pochonia chlamydosporia. Based on evolutionary and phylogenetic analyses, we have determined the relationships between the invertebrate-pathogenic fungi in Hypocreales. The invertebrate-pathogenic fungi in Hypocreales referred to in this paper form a monophyletic group sharing a most recent common ancestor. Our rps3 and trn gene order results also establish a foundation for further exploration of the evolutionary trajectory of the fungi in Hypocreales.
学科主题	Microbiology
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000350255100001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100927]
专题	蔬菜花卉研究所_植物保护研究室
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 2.Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China 3.Hunan Agr Univ, Key Lab Crop Germplasm Innovat & Utilizat Hunan P, Changsha 410128, Hunan, Peoples R China 4.Beijing Normal Univ, Coll Life Sci, Beijing 100875, Peoples R China
推荐引用方式 GB/T 7714	Lin, Runmao,Liu, Chichuan,Shen, Baoming,et al. Analysis of the complete mitochondrial genome of Pochonia chlamydosporia suggests a close relationship to the invertebrate-pathogenic fungi in Hypocreales[J]. BMC MICROBIOLOGY,2015,15:-.
APA	Lin, Runmao.,Liu, Chichuan.,Shen, Baoming.,Bai, Miao.,Ling, Jian.,...&Xie, Bingyan.(2015).Analysis of the complete mitochondrial genome of Pochonia chlamydosporia suggests a close relationship to the invertebrate-pathogenic fungi in Hypocreales.BMC MICROBIOLOGY,15,-.
MLA	Lin, Runmao,et al."Analysis of the complete mitochondrial genome of Pochonia chlamydosporia suggests a close relationship to the invertebrate-pathogenic fungi in Hypocreales".BMC MICROBIOLOGY 15(2015):-.