Identification and Characterization of the Gene CYP340W1 from Plutella xylostella and Its Possible Involvement in Resistance to Abamectin

doi:10.3390/ijms17030274

	Identification and Characterization of the Gene CYP340W1 from Plutella xylostella and Its Possible Involvement in Resistance to Abamectin
	Gao, Xue 1; Yang, Jiaqiang 1; Xu, Baoyun 1; Xie, Wen 1; Wang, Shaoli 1; Zhang, Youjun 1; Yang, Fengshan 2; Wu, Qingjun 1
刊名	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
	2016
卷号	17 期号:3 页码:-
关键词	Plutella xylostella overexpression P450 CYP340W1 RNA interference abamectin resistance
ISSN号	1422-0067
DOI	10.3390/ijms17030274
通讯作者	Gao, Xue
英文摘要	Abamectin has been used to control the diamondback moth, Plutella xylostella (P. xylostella), which is a major agricultural pest that can rapidly develop resistance against insecticides including abamectin. Although cytochrome P450 has been confirmed to play an important role in resistance in P. xylostella, the specific P450 genes associated with the resistance are unclear. The full-length cDNA of the cytochrome P450 gene CYP340W1 was cloned and characterized in the present study. The cDNA assembly yielded a sequence of 1929 bp, containing the open reading frame (ORF) 1491 bp and encodes a 496-amino acid peptide. CYP340W1 was expressed in all P. xylostella developmental stages but its expression level was highest in larvae and especially in the heads of larvae. The expression of CYP340W1 was significantly higher in an abamectin-resistant strain (ABM-R) than in its susceptible counterpart (ABM-S). In addition, expression of CYP340W1 was increased when the ABM-R strain was exposed to abamectin. When injected into third-stage ABM-R larvae, CYP340W1 dsRNA significantly reduced CYP340W1 expression at 6 h and reduced expression by 83% at 12 h. As a consequence of RNAi, the mortality of the injected abamectin-resistant larvae increased after a 48-h exposure to abamectin. The results indicate that the overexpression of CYP340W1 plays an important role in abamectin resistance in P. xylostella.
学科主题	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
语种	英语
出版者	MDPI AG
WOS记录号	WOS:000373712800136
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100839]
专题	蔬菜花卉研究所_植物保护研究室
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Plant Protect, Beijing 100081, Peoples R China 2.Univ Hei Longjiang, Coll Life Sci, Harbin 150000, Peoples R China
推荐引用方式 GB/T 7714	Gao, Xue,Yang, Jiaqiang,Xu, Baoyun,et al. Identification and Characterization of the Gene CYP340W1 from Plutella xylostella and Its Possible Involvement in Resistance to Abamectin[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2016,17(3):-.
APA	Gao, Xue.,Yang, Jiaqiang.,Xu, Baoyun.,Xie, Wen.,Wang, Shaoli.,...&Wu, Qingjun.(2016).Identification and Characterization of the Gene CYP340W1 from Plutella xylostella and Its Possible Involvement in Resistance to Abamectin.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,17(3),-.
MLA	Gao, Xue,et al."Identification and Characterization of the Gene CYP340W1 from Plutella xylostella and Its Possible Involvement in Resistance to Abamectin".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 17.3(2016):-.