心里美萝卜花青素合成酶基因RsANS克隆及花青素生物合成相关基因表达分析

doi:10.13430/j.cnki.jpgr.2016.05.015

	心里美萝卜花青素合成酶基因RsANS克隆及花青素生物合成相关基因表达分析
	孙玉燕; 段蒙蒙; 邱杨; 张晓辉; 沈镝; 王海平; 李锡香
刊名	植物遗传资源学报
	2016
卷号	17 期号:5 页码:889-896
关键词	心里美萝卜花青素合成酶基因克隆表达分析
ISSN号	1672-1810
DOI	10.13430/j.cnki.jpgr.2016.05.015
其他题名	Anthocyanidin Synthase Gene Cloning and Expression Analysis of Anthocyanidin Biosynthesis Related Genes in ‘Xinlimei’Radish(Raphanus sativus L.)
英文摘要	花青素合成酶(ANS,anthocyanidin synthase)是植物花青素合成的关键酶,催化无色花色素转变成有色花色素。本研究从心里美萝卜HX12Q-49中克隆获得了花青素合成酶基因RsANS(GenBank登录号: KR~262954)。该基因全长1137 bp,包含2个外显子和1个内含子;开放阅读框1071 bp,编码356个氨基酸。同源性分析显示RsANS蛋白与大白菜、甘蓝和芥菜ANS蛋白同源性较高。qRT-PCR表明RsANS在心里美萝卜5个不同发育时期均有表达,且在破肚期表达量最高。通过对花青素合成途径其他8个结构基因和3个调控基因表达进行分析,结果显示心里美萝卜中bHLH转录因子在不同发育时期的表达模式与RsANS、CHI、DFR和UFGT基本一致,即在破肚期的表达量最高; WD40转录因子的表达与CHS类似,其表达量在芽期、破肚期、膨大前期和膨大盛期逐渐上升,随后在成熟期下降。; Anthocyanin synthase(ANS) catalyzes the conversion of colorless leucoanthocyanins into colored anthocyanins, which is a key enzyme in the anthocyanin biosynthetic pathway. The RsANS gene(GenBank accession number: KR~262954) was isolated from‘Xinlimei’radish‘HX12Q-49’in present study. The full length of RsANS was 1137 bp and included 2 exons and 1 intron. The cDNA length of RsANS was 1071 bp and encoded a polypeptide of 356 amino acids. Homology analysis showed that RsANS had close relationship with ANS from Chinese cabbage, kohlrabi and mustard. qRT-PCR validation of RsANS revealed that it expressed at five developmental stages and reached the peak at cortex splitting stage. Furthermore, the expression level of another 8 functional genes and 3 regulatory genes at different developmental stages were investigated. The results showed that the expression pattern of bHLH transcription factor was similar to that of RsANS,CHI,DFR and UFGT,which peaked at cortex splitting stage. The expression pattern of WD40 transcription factor was similar to that of CHS,which gradually increased at seedling,cortex splitting,early expanding and rapid expanding stages, then decreased at mature stage.
学科主题	园艺
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101491]
专题	蔬菜花卉研究所_种质资源研究室
作者单位	中国农业科学院蔬菜花卉研究所, 北京, 100081
推荐引用方式 GB/T 7714	孙玉燕,段蒙蒙,邱杨,等. 心里美萝卜花青素合成酶基因RsANS克隆及花青素生物合成相关基因表达分析[J]. 植物遗传资源学报,2016,17(5):889-896.
APA	孙玉燕.,段蒙蒙.,邱杨.,张晓辉.,沈镝.,...&李锡香.(2016).心里美萝卜花青素合成酶基因RsANS克隆及花青素生物合成相关基因表达分析.植物遗传资源学报,17(5),889-896.
MLA	孙玉燕,et al."心里美萝卜花青素合成酶基因RsANS克隆及花青素生物合成相关基因表达分析".植物遗传资源学报 17.5(2016):889-896.