Proteomics-based identification of midgut proteins correlated with Cry1Ac resistance in Plutella xylostella (L.)

doi:10.1016/j.pestbp.2016.01.002

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	Proteomics-based identification of midgut proteins correlated with Cry1Ac resistance in Plutella xylostella (L.)
	Xia, Jixing 1,2; Guo, Zhaojiang 1; Yang, Zezhong 1; Zhu, Xun 1; Kang, Shi 1; Yang, Xin 1; Yang, Fengshan 3; Wu, Qingjun 1; Wang, Shaoli 1; Xie, Wen 1
刊名	PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
	2016
卷号	132 页码:108-117
关键词	Plutella xylostella Bacillus thuringiensis Cry1Ac resistance 2D-DIGE Ligand blotting
ISSN号	0048-3575
DOI	10.1016/j.pestbp.2016.01.002
通讯作者	Xia, Jixing
英文摘要	The diamondback moth, Plutella xylostella (L), is a worldwide pest of cruciferous crops and can rapidly develop resistance to many chemical insecticides. Although insecticidal crystal proteins (i.e., Cry and Cyt toxins) derived from Bacillus thuringiensis (Bt) have been useful alternatives to chemical insecticides for the control of P. xylostella, resistance to Bt in field populations of P. xylostella has already been reported. A better understanding of the resistance mechanisms to Bt should be valuable in delaying resistance development. In this study, the mechanisms underlying P. xylostella resistance to Bt Cry1Ac toxin were investigated using two-dimensional differential in-gel electrophoresis (2D-DIGE) and ligand blotting for the first time. Comparative analyses of the constitutive expression of midgut proteins in Cry1Ac-susceptible and -resistant P. xylostella larvae revealed 31 differentially expressed proteins, 21 of which were identified by mass spectrometry. Of these identified proteins, the following fell into diverse eukaryotic orthologous group (KOG) subcategories may be involved in Cry1Ac resistance in P. xylostella: ATP-binding cassette (ABC) transporter subfamily G member 4 (ABCG4), trypsin, heat shock protein 70 (HSP70), vacuolar H+-ATPase, actin, glycosylphosphatidylinositol anchor attachment 1 protein (GAA1) and solute carrier family 30 member 1 (SLC30A1). Additionally, ligand blotting identified the following midgut proteins as Cry1Ac-binding proteins in Cry1Ac-susceptible P. xylostella larvae: ABC transporter subfamily C member 1 (ABCC1), solute carrier family 36 member 1 (SLC36A1), NADH dehydrogenase iron-sulfur protein 3 (NDUFS3), prohibitin and Rap1 GTPase-activating protein 1. Collectively, these proteomic results increase our understanding of the molecular resistance mechanisms to Bt Cry1Ac toxin in P. xylostella and also demonstrate that resistance to Bt Cry1Ac toxin is complex and multifaceted. (C) 2016 Elsevier Inc. All rights reserved.
学科主题	Physiology
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000381777100016
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/105908]
专题	蔬菜花卉研究所_职能部门
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Plant Protect, 12 South St Zhong Guan Cun, Beijing 100081, Peoples R China 2.Heilongjiang Acad Agr Sci, Inst Plant Protect, Dept Biocontrol, Harbin 150080, Peoples R China 3.Heilongjiang Univ, Coll Life Sci, Key Lab Mol Biol Heilongjiang Prov, Harbin 150080, Peoples R China
推荐引用方式 GB/T 7714	Xia, Jixing,Guo, Zhaojiang,Yang, Zezhong,et al. Proteomics-based identification of midgut proteins correlated with Cry1Ac resistance in Plutella xylostella (L.)[J]. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY,2016,132:108-117.
APA	Xia, Jixing.,Guo, Zhaojiang.,Yang, Zezhong.,Zhu, Xun.,Kang, Shi.,...&Zhang, Youjun.(2016).Proteomics-based identification of midgut proteins correlated with Cry1Ac resistance in Plutella xylostella (L.).PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY,132,108-117.
MLA	Xia, Jixing,et al."Proteomics-based identification of midgut proteins correlated with Cry1Ac resistance in Plutella xylostella (L.)".PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 132(2016):108-117.