The Ph-3 gene from Solanum pimpinellifolium encodes CC-NBS-LRR protein conferring resistance to Phytophthora infestans

doi:10.1007/s00122-014-2303-1

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	The Ph-3 gene from Solanum pimpinellifolium encodes CC-NBS-LRR protein conferring resistance to Phytophthora infestans
	Zhang, Chunzhi 1; Liu, Lei 1; Wang, Xiaoxuan 1; Vossen, Jack 2; Li, Guangcun 3; Li, Tao 1; Zheng, Zheng 1; Gao, Jianchang 1; Guo, Yanmei 1; Visser, Richard G. F.2
刊名	THEORETICAL AND APPLIED GENETICS
	2014
卷号	127 期号:6 页码:1353-1364
ISSN号	0040-5752
DOI	10.1007/s00122-014-2303-1
通讯作者	Zhang, Chunzhi
英文摘要	Ph-3 is the first cloned tomato gene for resistance to late blight and encodes a CC-NBS-LRR protein.Late blight, caused by Phytophthora infestans, is one of the most destructive diseases in tomato. The resistance (R) gene Ph-3, derived from Solanum pimpinellifolium L3708, provides resistance to multiple P. infestans isolates and has been widely used in tomato breeding programmes. In our previous study, Ph-3 was mapped into a region harbouring R gene analogues (RGA) at the distal part of long arm of chromosome 9. To further narrow down the Ph-3 interval, more recombinants were identified using the flanking markers G2-4 and M8-2, which defined the Ph-3 gene to a 26 kb region according to the Heinz1706 reference genome. To clone the Ph-3 gene, a bacterial artificial chromosome (BAC) library was constructed using L3708 and one BAC clone B25E21 containing the Ph-3 region was identified. The sequence of the BAC clone B25E21 showed that only one RGA was present in the target region. A subsequent complementation analysis demonstrated that this RGA, encoding a CC-NBS-LRR protein, was able to complement the susceptible phenotype in cultivar Moneymaker. Thus this RGA was considered the Ph-3 gene. The predicted Ph-3 protein shares high amino acid identity with the chromosome-9-derived potato resistance proteins against P. infestans (Rpi proteins).
学科主题	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture ; AGRONOMY ; GENETICS & HEREDITY ; HORTICULTURE ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000336756200007
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100971]
专题	蔬菜花卉研究所_职能部门
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 2.Univ Wageningen & Res Ctr, Wageningen UR Plant Breeding, NL-6708 PB Wageningen, Netherlands 3.Shandong Acad Agr Sci, Inst Vegetables & Flowers, Jinan 250100, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Chunzhi,Liu, Lei,Wang, Xiaoxuan,et al. The Ph-3 gene from Solanum pimpinellifolium encodes CC-NBS-LRR protein conferring resistance to Phytophthora infestans[J]. THEORETICAL AND APPLIED GENETICS,2014,127(6):1353-1364.
APA	Zhang, Chunzhi.,Liu, Lei.,Wang, Xiaoxuan.,Vossen, Jack.,Li, Guangcun.,...&Du, Yongchen.(2014).The Ph-3 gene from Solanum pimpinellifolium encodes CC-NBS-LRR protein conferring resistance to Phytophthora infestans.THEORETICAL AND APPLIED GENETICS,127(6),1353-1364.
MLA	Zhang, Chunzhi,et al."The Ph-3 gene from Solanum pimpinellifolium encodes CC-NBS-LRR protein conferring resistance to Phytophthora infestans".THEORETICAL AND APPLIED GENETICS 127.6(2014):1353-1364.