Detoxification enzymes of Bemisia tabaci B and Q: biochemical characteristics and gene expression profiles

doi:10.1002/ps.3751

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	Detoxification enzymes of Bemisia tabaci B and Q: biochemical characteristics and gene expression profiles
	Guo, Litao; Xie, Wen; Wang, Shaoli; Wu, Qingjun; Li, Rumei; Yang, Nina; Yang, Xin; Pan, Huipeng; Zhang, Youjun
刊名	PEST MANAGEMENT SCIENCE
	2014
卷号	70 期号:10 页码:1588-1594
关键词	Bemisia tabaci detoxification enzymes quantitative real-time PCR analysis cytochrome-P450-dependent monooxygenase carboxylesterase glutathione S-transferase
ISSN号	1526-498X
DOI	10.1002/ps.3751
通讯作者	Guo, Litao
英文摘要	BACKGROUND: The sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is one of the most invasive and destructive pests of field crops worldwide. The sibling species B and Q are the two most damaging members of the B. tabaci species complex. That Q is more resistant than B to many insecticides has been well documented. Over the last decade, Q has gradually displaced B and has become the dominant form of B. tabaci in field agricultural systems inmost parts of China. To help understand the differences in insecticide resistance, the activities and gene expression profiles of detoxification enzymes in B. tabaci B and Q were investigated.RESULTS: The activity of P450 towards 7-ethoxycoumarin was significantly higher (1.46-fold higher) in Q than in B. The expression of 43 of 65 P450 genes was higher (>1-fold) in Q than in B, and expression for eight P450 genes was more than 50-fold greater in Q than in B. The increased expression of selected P450 genes in Q relative to B was confirmed with two other B strains and two other Q strains. On the other hand, carboxylesterase (CarE) activity was significantly lower (0.71-fold lower) in Q than in B; the K-m value of CarE was significantly lower in B than in Q, but the opposite was true for the V-max value of CarE. Glutathione S-transferase activity and values of K-m and V-max did not differ between B and Q.CONCLUSION: Enhanced metabolic detoxification of insecticides by P450s may be an important reason why B. tabaci Q is more resistant than B. tabaci B to insecticides. (C) 2014 Society of Chemical Industry
学科主题	Agronomy ; Entomology
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000342621800020
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100952]
专题	蔬菜花卉研究所_职能部门
作者单位	Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Plant Protect, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Guo, Litao,Xie, Wen,Wang, Shaoli,et al. Detoxification enzymes of Bemisia tabaci B and Q: biochemical characteristics and gene expression profiles[J]. PEST MANAGEMENT SCIENCE,2014,70(10):1588-1594.
APA	Guo, Litao.,Xie, Wen.,Wang, Shaoli.,Wu, Qingjun.,Li, Rumei.,...&Zhang, Youjun.(2014).Detoxification enzymes of Bemisia tabaci B and Q: biochemical characteristics and gene expression profiles.PEST MANAGEMENT SCIENCE,70(10),1588-1594.
MLA	Guo, Litao,et al."Detoxification enzymes of Bemisia tabaci B and Q: biochemical characteristics and gene expression profiles".PEST MANAGEMENT SCIENCE 70.10(2014):1588-1594.